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Author:   Kenneth C. Keiler
Publisher:   Humana Press Inc.
Imprint:   Humana Press Inc.
Edition:   2012 ed.
Volume:   905
Dimensions:   Width: 17.80cm , Height: 2.10cm , Length: 25.40cm
Weight:   0.852kg
ISBN:  

9781617799488

ISBN 10:   1617799483
Pages:   333
Publication Date:   28 June 2012
Audience:   Professional and scholarly ,  Professional & Vocational
Format:   Hardback
Publisher's Status:   Active
Availability:   Manufactured on demand  
We will order this item for you from a manufactured on demand supplier.

Table of Contents

 Bioinformatic Discovery of Bacterial Regulatory RNAs using SIPH.-How to Identify CRISPRs in Sequencing Data.-A Strategy for Identifying Non-coding RNAs using Whole-genome Tiling Arrays.-Genetic Screens to Identify Bacterial sRNA Regulators.-Competition Assays Using Barcoded Deletion Strains to Gain Insight Into Small RNA Function.-Use of Semi-quantitative Northern Blot Analysis to Determine Relative Quantities of Bacterial CRISPR Transcripts.-RNA Visualization in Bacteria by Fluorescence in Situ Hybridization.-RNA Structure Prediction: An Overview of Methods.-Crystallization of RNA-Protein Complexes: From Synthesis and Purification of Individual Components to Crystals.-Analysis of RNA Folding and Ligand Binding by Conventional and High-Throughput Calorimetry.-Use of Aptamer Tagging to Identify in vivo Protein Binding Partners of Small Regulatory RNAs.-Gel Mobility Shift Assays to Detect Protein-RNA Interactions.-RNase Footprinting of Protein Binding Sites on an mRNA Target of Small RNAs.-Computational Identification of sRNA Targets.-Detection of sRNA-mRNA Interactions by Electrophoretic Mobility Shift Assay.-Activity of Small RNAs on the Stability of Targeted mRNAs in vivo.-Native Gel Electrophoresis to Study the Binding and Release of RNA Polymerase by 6S RNA.-Ribosome Purification Approaches for Studying Interactions of Regulatory Proteins and RNAs with the Ribosome.-Analysis of Aminoacyl- and Peptidyl-tRNAs by Gel Electrophoresis.- In vitro trans-translation Assays.

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