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Author:   Michael D. Manson
Publisher:   Humana Press Inc.
Imprint:   Humana Press Inc.
Edition:   1st ed. 2018
Volume:   1729
Weight:   0.978kg
ISBN:  

9781493975761

ISBN 10:   1493975765
Pages:   389
Publication Date:   11 February 2018
Audience:   Professional and scholarly ,  Professional & Vocational
Format:   Hardback
Publisher's Status:   Active
Availability:   Manufactured on demand  
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Table of Contents

The Diversity of Bacterial Chemosensing.- Transmembrane Signal Transduction in Bacterial Chemosensing.- Two Spatial Chemotaxis Assays: The Nutrient-Depleted Chemotaxis Assay and the Agarose-Plug-Bridge Assay.- Quantification of Bacterial Chemotaxis Responses at the Mouths of Hydrogel Capillaries.- A Static Microfluidic Device for Investigating the Chemotaxis Response to Stable, Non-Linear Gradients.- Visualizing Chemoattraction of Planktonic Cells to a Biofilm.- Labeling Bacterial Flagella with Fluorescent Dyes.- All-Codon Mutagenesis for Structure-Function Studies of Chemotaxis Signaling Proteins.- Mutational Analysis of Binding Protein/Chemoreceptor Interactions.- In Vitro Assay for Measuring Receptor-Kinase Activity in the Bacillus subtilis Chemotaxis Pathway.- FRET Analysis of the Chemotaxis Pathway Response.- Monitoring Two-Component Sensor Kinases with a Chemotaxis Signal Readout.- Analyzing Protein Domain Interactions in Chemoreceptors by In Vivo PEGylation.- Tuning Chemoreceptor Signaling by Positioning Aromatic Residues at the Lipid-Aqueous Interface.- Analyzing Chemoreceptor Interactions In Vivo with the Tri-Functional Crosslinker TMEA.- Use of Cryo-EM to Study the Structure of Chemoreceptor Arrays In Vivo.- Visualizing Chemoreceptor Arrays in Bacterial Minicells by Cryo-Electron Tomography and Subtomogram Analysis.- Bacterial Chemoreceptor Imaging at High Spatio-Temporal Resolution Using Photoconvertible Fluorescent Proteins.- Imaging of Single Dye-Labeled Chemotaxis Proteins in Live Bacteria Using Electroporation.- Fluorescence Anisotropy to Detect In Vivo Stimulus-Induced Changes in Chemoreceptor Packing.- Chemotaxis to Atypical Chemoattractants by Soil Bacteria.- Screening Chemoreceptor–Ligand Interactions by High-Throughput Thermal-Shift Assays.- High-Throughput Screening to Identify Chemoreceptor Ligands.- Identification of Specific Ligands for Sensory Receptors by Small-Molecule Ligand Arrays and Surface Plasmon Resonance.- Fluorescence Measurement of Kinetics of CheY Autophosphorylation with Small Molecule Phosphodonors.- Synthesis of a Stable Analog of the Phosphorylated Form of CheY: Phosphono-CheY.- Quantitative Modeling of Flagellar Motor-Mediated Adaptation.- Molecular Modeling of Chemoreceptor:Ligand Interactions.- Phylogenetic and Protein Sequence Analysis of Bacterial Chemoreceptors.

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