Astrocytes: Methods and Protocols

Author:   Barbara Di Benedetto
Publisher:   Springer-Verlag New York Inc.
Edition:   Second Edition 2025
ISBN:  

9781071643686


Pages:   288
Publication Date:   21 March 2026
Format:   Paperback
Availability:   Manufactured on demand   Availability explained
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Astrocytes: Methods and Protocols


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Author:   Barbara Di Benedetto
Publisher:   Springer-Verlag New York Inc.
Imprint:   Humana
Edition:   Second Edition 2025
ISBN:  

9781071643686


ISBN 10:   1071643681
Pages:   288
Publication Date:   21 March 2026
Audience:   Professional and scholarly ,  Professional & Vocational
Format:   Paperback
Publisher's Status:   Active
Availability:   Manufactured on demand   Availability explained
We will order this item for you from a manufactured on demand supplier.

Table of Contents

Immunofluorescence Protocol to Label Astrocytes from Diverse Model and Non-Model Mammals.- Retro-Orbital Delivery of AAVs for CNS-Wide Astrocyte Targeting.- An Empirical-Theoretical Approach to Determine Astroglial Potassium upon Ischemic Stress.- Quantification of Astrocytic Sodium Signals Using Fluorescence Lifetime Imaging (FLIM).- pH Sensor-Based Engulfment Assays to Measure Phagocytic Activity of Astrocytes.- An Astrocyte Transplantation Method to Investigate Astrocyte Development and Diversity in the CNS.- Primary CNS Cultures with Mixed Neural Cell Types to Study Correlative Effects of High Glucocorticoids on Astrocytes, Oligodendrocytes, and Myelination Markers.- Postnatal Development of Perivascular Astrocytic Processes and Detection of Local mRNA and Translation.- Imaging Techniques for the Analysis of the Blood-Brain Barrier and Gliovascular Unit Dysfunction.- Assessment of Blood-Brain Barrier Integrity Using Dyes and Tracers.- Investigating the Pathology-Related Astroglial Plasticity in the Human Cerebral Cortex.- Super-Resolution Analysis of Astrocyte Morphology Using Expansion Microscopy.- Astrocyte Morphological Adaptations in a Mouse Model of Social Anxiety Disorder.- An In Vitro Assay to Measure Astrocyte-Dependent Synaptic Phagocytosis in Health and Major Depressive Disorder.- Cortical Organotypic Brain Slice Cultures to Examine Sex- and Age-Dependent Astrocyte-Mediated Synaptic Phagocytosis.- Development and Validation of Stress Paradigms to Study Astrocytes in Rodent Models of Stress Disorders.- Cellular Investigation of Astrocytic Neuroendocrine Responses to Stress.- Morphological Investigation of Astrocytic Responses to Stress.- Functional Evaluation of Astrocytic Responses to Stress.- Unveiling Ketamine's Influence on Astrocytic Kir4.1 Channels through Multi-Modal Analysis: Confocal Microscopy, Immunocytochemistry, Fluorescence Analysis, and Electrophysiology.- Revealing and Characterizing Astrocytic Atrophy in an Animal Model of Depression.

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