Overview

Arthritis Research: Methods and Protocols, Second Edition expands upon the first edition to present new and current techniques for the research of arthritis and related conditions. A compendium of leaders in the field contribute chapters that cover practical research methods such as the intravital multiphoton microscopy technique, techniques for evaluating exhausted CD8 T cell and for studying nucleic acid sensors and their effects, methods for in vivo tetracycline-controlled transgenic mice and T cell receptor transgenic mice, protocols to detect V(D)J recombination products and microRNA, and the technique to make bleomycin-induced dermal fibrosis. Written in the successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible protocols, and notes on troubleshooting and avoiding known pitfalls.   Authoritative and easily accessible, Arthritis Research: Methods and Protocols, Second Edition will serve both professionals and novices with state-of-the-art techniques pertaining to this fascinating research field.

Full Product Details

Author:   Shunichi Shiozawa
Publisher:   Humana Press Inc.
Imprint:   Humana Press Inc.
Edition:   Softcover reprint of the original 2nd ed. 2014
Volume:   1142
Dimensions:   Width: 17.80cm , Height: 1.20cm , Length: 25.40cm
Weight:   4.148kg
ISBN:  

9781493953936

ISBN 10:   1493953931
Pages:   204
Publication Date:   23 August 2016
Audience:   Professional and scholarly ,  Professional & Vocational
Format:   Paperback
Publisher's Status:   Active
Availability:   Manufactured on demand  
We will order this item for you from a manufactured on demand supplier.

Table of Contents

Intravital Multiphoton Microscopy for Dissecting Cellular Dynamics in Arthritic Inflammation and Bone Destruction.- Monitoring Multifunctionality of Immune-Exhausted CD8 T in Cancer Patients.- Characterization of Innate Immune Signalings Stimulated by Ligands for Pattern Recognition Receptors.- Principled for the Use of in vivo Transgene Techniques: Overview and an Introductory Practical Guide for the Selection of Tetracycline-Controlled Transgenic Mice.- Unraveling Autoimmunity with the Adoptive Transfer of T Cells from TCR-Transgenic Mice.- In Vivo Cell Transfer Assay to Detect Autoreactive T Cell Subsets.- Characterization of microRNAs and Their Targets.- Studies on the T Cell Receptor (TCR) Revision of Autoantibody-Inducing CD4T (aiCD4 T) Cell.- Basic Techniques for Studies of iNKT Cells and MAIT Cells.- Induction of de novo Autoimmune Disease in Normal Mice Upon Repeated Immunization with Antigen.- Mouse Models of Experimental Dermal Fibrosis: The Bleomycin-Induced Dermal Fibrosis.- Screening for Novel Serum Biomarker for Monitoring Disease Activity in Rheumatoid Arthritis Using iTRAQ Technology Based Quantitative Proteomic Approach.- Genome-Wide Genetic Study in Autoimmune Disease Prone Mice.- Bayesian Systems-Based Genetic Association Analysis with Effect Strength Estimation and Omic Wide Interpretation: A Case Study in Rheumatoid Arthritis.- Sample Processing, Protocol and Statistical Analysis of the Time of Flight Secondary Ion Mass Spectrometry (ToF-SIMS) of Protein, Cell and Tissue Samples.- Label-Free Imaging ofAdipogenesis by Coherent Anti-Stokes Raman Scattering Microscopy.

Reviews

From the book reviews: This book offers insights and protocols regarding 16 assays used by scientists studying the workings of the immune system, particularly with respect to autoimmunity. ... It is intended for scientists who are interested in reviewing the theory behind a technique and/or tips for conducting it. Clinicians trying to gain a better understanding of scientific techniques as they are appraising the primary literature in their field also could presumably use it. (Tiphanie Vogel, Doody's Book Reviews, September, 2014)

From the book reviews: This book offers insights and protocols regarding 16 assays used by scientists studying the workings of the immune system, particularly with respect to autoimmunity. ... It is intended for scientists who are interested in reviewing the theory behind a technique and/or tips for conducting it. Clinicians trying to gain a better understanding of scientific techniques as they are appraising the primary literature in their field also could presumably use it. (Tiphanie Vogel, Doody's Book Reviews, September, 2014)

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