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Author:   Rajni Hatti-Kaul
Publisher:   Humana Press Inc.
Imprint:   Humana Press Inc.
Edition:   2000 ed.
Volume:   11
Dimensions:   Width: 15.20cm , Height: 3.00cm , Length: 22.90cm
Weight:   0.855kg
ISBN:  

9780896035416

ISBN 10:   0896035417
Pages:   454
Publication Date:   21 February 2000
Audience:   College/higher education ,  Professional and scholarly ,  Undergraduate ,  Professional & Vocational
Format:   Hardback
Publisher's Status:   Active
Availability:   In Print  
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Table of Contents

Preparation and Characterization of Aqueous Two-Phase Systems.- Aqueous Two-Phase Systems.- The Phase Diagram.- Preparation of Aqueous Two-Phase Systems.- Measurement of Some Physical Properties of Aqueous Two-Phase Systems.- Partitioning Techniques.- Single-Step Partitioning in Aqueous Two-Phase Systems.- Partition by Countercurrent Distribution (CCD).- Liquid-Liquid Partition Chromatography (LLPC).- Partitioning of Soluble Molecules and Particulates.- Metal Ion Separations in Aqueous Biphasic Systems and with ABEC™ Resins.- Partition of Amino Acids and Peptides in Aqueous Two-Phase Systems.- Predicting Partition Coefficients of Small Solutes Based on Hydrophobicity.- Eukaryotic Cell Partition.- Concentration and Purification of Viruses.- Isolation of Plant Plasma Membranes and Production of Inside-Out Vesicles.- Isolation of Inside-Out Thylakoid Vesicles.- Two-Phase Partitioning as a Method for Isolation of Tight Plasma Membrane Vesicles from Saccharomyces cerevisiae and from Chlamydomonas reinhardtii.- Purification of Cyanobacterial Thylakoid, Plasma, and Outer Membranes by Two-Phase Partitioning.- Purification of Plasma Membranes by Affinity Partitioning.- Studying the Influence of Salts on Partitioning of Proteins.- Partitioning of Chemically Modified Proteins.- Detection and Analysis of Interactions by Two-Phase Partition.- Cryopartitioning in Two-Phase Systems Containing Organic Solvents.- Isolation and Purification of Proteins by Two-Phase Extraction.- Optimization of Extractions in Aqueous Two-Phase Systems.- Two-Phase Extraction of Proteins from Cell Debris.- Aqueous Two-Phase Extraction of Proteins from Animal Tissue.- Chaotropic Aqueous Two-Phase Systems.- Temperature-Induced Phase Partitioning for Protein Purification.- Extraction of Amphiphilic ProteinsUsing Detergent-Based Aqueous Two-Phase Systems.- Use of Charged PEG and Dextran Derivatives for Biomolecule Partitioning.- Affinity Partitioning Using Poly(ethylene glycol) with Covalently Coupled Hydrophobic Groups.- Dye-Ligand Affinity Partitioning of Proteins.- Metal Affinity Protein Partitioning.- Recovery of Proteins and Phase Components.- Combination of Extraction with Adsorption for Protein Purification.- Integration of Extraction with Affinity Precipitation.- Affinity Partitioning Using Magnetic Two-Phase Systems.- 36 Large-Scale Extraction of Proteins.- Bioconversions in Two-Phase Systems.- Extractive Bioconversion in Aqueous Two-Phase Systems.- Enzyme Reaction in Polymer-Polymer Organic Solvent Two-Phase Systems.

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