Overview

Volume 322 of Methods in Enzymology is dedicated to apoptosis. Major topics covered include measuring apoptosis and apoptosis-induced endonucleases, measuring apoptosis in lower organisms, proteases involved in apoptosis and their inhibitors, cell free systems for monitoring steps in apoptosis pathways, mitochondria and apoptosis, bCl-2 family proteins, and studying receptors and signal transduction events implicated in cell survival and cell death. The critically acclaimed laboratory standard for more than forty years, Methods in Enzymology is one of the most highly respected publications in the field of biochemistry. Since 1955, each volume has been eagerly awaited, frequently consulted, and praised by researchers and reviewers alike. Now with more than 300 volumes (all of them still in print), the series contains much material still relevant today--truly an essential publication for researchers in all fields of life sciences.

Full Product Details

Author:   John N. Abelson (California Institute of Technology, Division of Biology, Pasadena, U.S.A.) ,  Melvin I. Simon (The Salk Institute, La Jolla, CA, USA) ,  John C. Reed (The Burnham Institute, La Jolla, California, U.S.A.) ,  John N. Abelson (California Institute of Technology, Division of Biology, Pasadena, U.S.A.)
Publisher:   Elsevier Science Publishing Co Inc
Imprint:   Academic Press Inc
Volume:   v. 322
Dimensions:   Width: 15.20cm , Height: 3.80cm , Length: 22.90cm
Weight:   0.940kg
ISBN:  

9780121822231

ISBN 10:   0121822230
Pages:   569
Publication Date:   19 July 2000
Audience:   Professional and scholarly ,  Professional & Vocational
Format:   Hardback
Publisher's Status:   Unknown
Availability:   In Print  
Limited stock is available. It will be ordered for you and shipped pending supplier's limited stock.

Table of Contents

Detection of DNA Cleavage in Apoptotic Cells Detection of Apoptosis by Annexin V Labeling Analysis of Apoptotic Cells by Flow and Laser Scanning Cytometry Quantitative Measurement of Apoptosis Induced by Cytotoxic T Lymphocytes Apoptotic Nuclease Assays Analysis of Programmed Cell Death and Apoptosis in Drosophila Analysis of Programmed Cell Death in the Nematode Caenorhabditis elegans Caspase Assays Determination of Caspase Specificities Using a Peptide Combinatorial Library Criteria for Identifying Authentic Caspase Substrates during Apoptosis Purification and Use of Granzyme B Viral Caspase Inhibitors CrmA and p35 Purification and Use of Recombinant Inhibitor of Apoptosis Proteins as Caspase Inhibitors Monitoring Activity of Caspases and Their Regulators in Yeast Saccharomyces cerevisiae In Vitro Assays for Caspase-3 Activation and DNA Fragmentation Cell-Free Apoptosis in Xenopus laevis Egg Extracts Cytofluorometric Quantitation of Nuclear Apoptosis Induced in a Cell-Free System Purification of Mitochondria for Apoptosis Assays Quantitation of Mitochondrial Transmembrane Potential in Cells and in Isolated Mitochondria Nitrogen Cavitation for Cell Disruption to Obtain Mitochondria from Cultured Cells Apoptosis-Related Activities Measured with Isolated Mitochondria and Digitonin-Permeabilized Cells Assays for Cytochrome c Release from Mitochondria during Apoptosis Purification and Liposomal Reconstitution of Permeability Transition Pore Complex Monitoring Interactions of Bcl-2 Family Proteins in 96-Well Plate Assays Analysis of Dimerization of Bcl-2 Family Proteins by Surface Plasmon Resonance Measuring Pore Formation by Bcl-2 Family Proteins Assays for Studying Bax-Induced Lethality in the Yeast Saccharomyces cereνisiae Exploiting the Utility of Yeast in the Context of Programmed Cell Death Production of Recombinant TRAIL and TRAIL Receptor: Fc Chimeric Proteins Expression of Lymphotoxins and Their Receptor–Fc Fusion Proteins by Baculovirus Analysis of the CD95 (APO-1/Fas) Death-Inducing Signaling Complex by High-Resolution Two-Dimensional Gel Electrophoresis Measurement of Ceramide Levels by the Diacylglycerol Kinase Reaction and by High-Performance Liquid Chromatography–Fluorescence Spectrometry Measurement of Ceramide Synthase Activity Measurement of Sphingomyelinase Activity Assays for JNK and p38 Mitogen-Activated Protein Kinases Assaying for IκB Kinase Activity Assays for Akt Measurement of Cellular Oxidation, Reactive Oxygen Species, and Antioxidant Enzymes during Apoptosis Volume Regulation and Ion Transport during Apoptosis Assays for Transglutaminases in Cell Death Anoikis Transient Transfection Assay of Cell Death Genes Sindbis Virus Vector System for Functional Analysis of Apoptosis Regulators Transduction of Full-Length Tat Fusion Proteins Directly into Mammalian Cells: Analysis of T Cell Receptor Activation-Induced Cell Death Author Index Subject Index

Reviews

Praise for the Series The Methods in Enzymology series represents the gold-standard. --NEUROSCIENCE Incomparably useful. --ANALYTICAL BIOCHEMISTRY It is a true 'methods' series, including almost every detail from basic theory to sources of equipment and reagents, with timely documentation provided on each page. --BIO/TECHNOLOGY The series has been following the growing, changing and creation of new areas of science. It should be on the shelves of all libraries in the world as a whole collection. --CHEMISTRY IN INDUSTRY The appearance of another volume in that excellent series, Methods in Enzymology, is always a cause for appreciation for those who wish to successfully carry out a particular technique or prepare an enzyme or metabolic intermediate without the tiresome prospect of searching through unfamiliar literature and perhaps selecting an unproven method which is not easily reproduced. --AMERICAN SOCIETY OF MICROBIOLOGY NEWS If we had some way to find the work most often consulted in the laboratory, it could well be the multi-volume series Methods in Enzymology...a great work. --ENZYMOLOGIA A series that has established itself as a definitive reference for biochemists. --JOURNAL OF CHROMATOGRAPHY

Author Information

Tab Content 6

Author Website:  

Customer Reviews

Recent Reviews

No review item found!

Add your own review!

Countries Available

All regions