Overview

This text is designed to provide conceptual outlines and detailed procedures for basic and advanced studies of cell death by apoptosis. Chapters on the recognition of apoptosis as distinguished from necrosis and nonspecific cell DNA damage, are followed by a systematic examination of the established and the principal novel methodologies utilized by some leading laboratories conducting research on apoptosis. The organization is on the lines of signalling for apoptosis, the apoptotic cascade, and the execution of apoptosis. A wide variety of procedures are provided which will enable the reader to participate in cutting-edge research.

Full Product Details

Author:   George Studzinski (Professor of Pathology and Laboratory Medicine, New Jersey Medical School)
Publisher:   Oxford University Press
Imprint:   Oxford University Press
Volume:   216
Dimensions:   Width: 15.70cm , Height: 1.60cm , Length: 23.40cm
Weight:   0.423kg
ISBN:  

9780199637850

ISBN 10:   0199637857
Pages:   266
Publication Date:   09 December 1999
Audience:   Professional and scholarly ,  Professional & Vocational
Format:   Paperback
Publisher's Status:   Out of Print
Availability:   Out of stock  

Table of Contents

Preface 1: Studzinski: Overview of Apoptosis 2: Wilson and Potten: The Morphological Recognition of Apoptosis 3: Yoshida, Shao and Pommier: Assessment of DNA Damage in Apoptosis 4: Darzynkiewicz, Bedner and Li: Analysis of Cell Death by Flow and Laser Scanning Cytometry 5: Naval and Anel: Cell-mediated Cytotoxicity and Cell Death Receptors 6: Jenkins and Hannun: Sphingolipids as Messengers of Cell Death 7: Van Engeland et al: Cytochemical Detection of Cyto- and Nucleoskeletal Changes During Apoptosis 8: Costa-Pereira and Cotter: 9: Reed et al: Methods of Measuring Bcl-2 Family Proteins and Their Functions 10: Blajeski and Kaufmann: Methods for Detecting Proteolysis During Apoptosis in Intact Cells Index

Reviews

The major strength of this book is the wealth of apaptosis-associated protocols provided by the authors ... a significant addition to the apaptosis literature and particularly valuable for mammalian cell biologists Trends in Cell Biology

Ten chapters by contributors from leading laboratories globally provide a comprehensive guide to the latest techniques for studying cell death by apoptosis. Early chapters distinguish apoptosis for normal cell population control from necrosis and non-specific cell DNA damage. Later chapters cover step-by-step use of key investigation methods, particularly signaling, the apoptotic cascade, and the execution of apoptosis. Topics include: assessment of DNA damage, analysis by flow and laser-scanning cytometry, sphingolipids as messengers of cell death, and associated metabolic alterations. Includes microscopy images, and a list of US and UK lab suppliers. The editor is affiliated with the New Jersey Medical School. --SciTech Book News<br>

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