Overview

Full Product Details

Author:   Ronald P. van Rij
Publisher:   Humana Press Inc.
Imprint:   Humana Press Inc.
Edition:   Softcover reprint of the original 1st ed. 2011
Volume:   721
Weight:   1.097kg
ISBN:  

9781493958252

ISBN 10:   1493958259
Pages:   425
Publication Date:   23 August 2016
Audience:   Professional and scholarly ,  Professional & Vocational
Format:   Paperback
Publisher's Status:   Active
Availability:   Manufactured on demand  
We will order this item for you from a manufactured on demand supplier.

Table of Contents

Defense and counter-defense in the RNAi-based antiviral immune system in insects.- RNAi and cellular miRNAs in infections by mammalian viruses.- Viral miRNAs.- Progress in RNAi-based antiviral therapeutics.- Chemical modification of small interfering RNA.- Viral small RNA cloning and sequencing.- Visitor, an informatic pipeline for analysis of viral siRNA sequencing datasets.- Computational prediction of viral miRNAs.- Detection of Viral microRNAs by Northern Blot Analysis.- Detection of viral microRNA with S1 Nuclease Protection assay.- Characterization of RISC associated adenoviral small RNAs.- Identification of Viral Suppressors of RNAi by a Reporter Assay in Drosophila S2 Cell Culture.- Dicer assay in Drosophila S2 cell extract.- Slicer activity in Drosophila melanogaster S2 extract.- Gel Mobility Shift Assays for RNA-binding viral RNAi suppressors.- dsRNA uptake in adult Drosophila.- Design of small interfering RNAs for antiviral applications.- RNAi inducing lentiviral vectors for anti-HIV-1 gene therapy.- Production of multicopy shRNA lentiviral vectors for antiviral therapy.- Intranasal delivery of antiviral siRNA.- Antibody mediated delivery of siRNAs for anti-HIV therapy.- Aptamer-targeted RNAi for HIV-1 therapy.- RNAi screening for host factors involved in viral infection using Drosophila cells.- Genome-wide RNAi Screen for ViralReplication in Mammalian Cell Culture.- RNAi screening in mammalian cells to identify novel host cell molecules involved in the regulation of viral infections.

Reviews

From the reviews: This well-illustrated book details the necessary protocols for the analysis of viral small RNAs and natural antiviral RNAi responses as well as the development and optimization of RNAi-based antiviral drugs. The book is intended for both novice and experienced researchers ... . It also will be of immense help to graduate students, physicians who treat patients with infectious diseases, and other researchers interested in the field of antiviral RNAi. ... a well-illustrated and authoritative resource on important basic concepts in the field of antiviral RNAi. (Omer Iqbal, Doody's Review Service, July, 2011)

From the reviews: This well-illustrated book details the necessary protocols for the analysis of viral small RNAs and natural antiviral RNAi responses as well as the development and optimization of RNAi-based antiviral drugs. The book is intended for both novice and experienced researchers ... . It also will be of immense help to graduate students, physicians who treat patients with infectious diseases, and other researchers interested in the field of antiviral RNAi. ... a well-illustrated and authoritative resource on important basic concepts in the field of antiviral RNAi. (Omer Iqbal, Doody's Review Service, July, 2011)

From the reviews: “This well-illustrated book details the necessary protocols for the analysis of viral small RNAs and natural antiviral RNAi responses as well as the development and optimization of RNAi-based antiviral drugs. The book is intended for both novice and experienced researchers … . It also will be of immense help to graduate students, physicians who treat patients with infectious diseases, and other researchers interested in the field of antiviral RNAi. … a well-illustrated and authoritative resource on important basic concepts in the field of antiviral RNAi.” (Omer Iqbal, Doody’s Review Service, July, 2011)

Author Information

Tab Content 6

Author Website:  

Customer Reviews

Recent Reviews

No review item found!

Add your own review!

Countries Available

All regions