Overview

This detailed volume aims to elucidate the molecular mechanisms that underlie antifungal resistance. The book highlights methods to identify and characterize antifungal activity, to define and characterize strains with altered responses to antifungal drugs, to investigate the genetic and molecular mechanisms of these alterations of antifungal drug susceptibility, and, finally, to approach the study of these processes in animal models of fungal infection. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step and readily reproducible laboratory protocols, as well as tips on troubleshooting and avoiding known pitfalls.  Authoritative and practical, Antifungal Drug Resistance: Methods and Protocols serves as an ideal resource for the field and a guide for investigators to study this important translational aspect of fungal biology.

Full Product Details

Author:   Damian J. Krysan ,  W. Scott Moye-Rowley
Publisher:   Springer-Verlag New York Inc.
Imprint:   Springer-Verlag New York Inc.
Edition:   1st ed. 2023
Volume:   2658
Weight:   0.693kg
ISBN:  

9781071631546

ISBN 10:   1071631543
Pages:   250
Publication Date:   07 April 2023
Audience:   Professional and scholarly ,  Professional & Vocational
Format:   Hardback
Publisher's Status:   Active
Availability:   Manufactured on demand  
We will order this item for you from a manufactured on demand supplier.

Table of Contents

Part I: Identification and Characterization of Molecules with Antifungal Activity   1. Antifungal Susceptibility of Yeasts and Filamentous Fungi by CLSI Broth Microdilution Testing             Nathan P. Wiederhold   2. Luciferase-Based High Throughput Screen with Aspergillus fumigatus to Identify Antifungal Small Molecules             Timothy J. Opperman, Sourabh Dhingra, Cecilia Gutierrez, Steven M. Kwasny, and Robert A. Cramer   3. A Dual-Readout High Throughput Screening Assay for Small Molecules Active Against Aspergillus fumigatus             Sarah R. Beattie and Damian J. Krysan   4. Antifungal Susceptibility Testing and Drug Discovery in the Dimorphic Fungus Histoplasma capsulatum             Chad A. Rappleye   5. A 384-Well Microtiter Plate Model for Candida Biofilm Formation and Its Application to High Throughput Screening             Olabayo H. Ajetunmobi, Gina Wall, Bruna V. Bonifacio, Daniel Montelongo-Jauregui, and Jose L. Lopez-Ribot   Part II: Identification and Characterization of Fungal Strains with Altered Drug Susceptibility   6. Protocols for Measuring Tolerant and Heteroresistant Drug Responses of Pathogenic Yeasts             Naomi Lyons and Judith Berman   7. Genome-Wide Identification of Variants Associated with Antifungal Drug Resistance             Aina Martinez-Zurita and Christina A. Cuomo   8. Copy Number Variation and Allele Ratio Analysis in Candida ablicans Using Whole Genome Sequencing Data             Robert T. Todd and Anna Selmecki   9. Drug Target Elucidation through Isolation and Analysis of Drug-Resistant Mutants in Cryptococcus neoformans             Michael J. Hoy and Joseph Heitman   10. Chemical-Genetic Approaches for Exploring Mode of Action of Antifungal Compounds in the Fungal Pathogen Candida albicans             Nicole Robbins, Troy Ketela, Sang Hu Kim, and Leah E. Cowen   Part III: Characterization of the Genetic and Molecular Mechanisms of Antifungal Resistance   11. Characterizing Candida glabrata Pdr1, a Hyperactive Transcription Factor Involved in Azole Resistance             Lucia Simonicova and W. Scott Moye-Rowley   12. Heterologous Expression of Candida Antifungal Target Genes in the Model Organism Saccharomyces cerevisiae             Kelley R. Healey and Ashutosh Singh   13. Conditional Protein Depletion in the Analysis of Antifungal Drug Resistance in Candida glabrata             Thomas P. Conway and W. Scott Moye-Rowley   14. Transport Across Membranes: Techniques for Measuring Efflux in Fungal Cells             Brooke D. Esquivel and Theodore C. White   15. Transport Across Membranes: Techniques for Measuring Drug Import in Fungal Cells             Brooke D. Esquivel and Theodore C. White   Part IV: Characterization of Antifungal Drug Resistance Using Animal Infection Models   16. Analysis of Candida Antifungal Resistance Using Animal Infection Models             David R. Andes and Jeniel E. Nett   17. Using Bioluminescence to Image Candida glabrata Urinary Tract Infections in Mice             Sanne Schrevens, Riccardo Torelli, Maurizio Sanguinetti, and Dominque Sanglard

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