Overview

Recombinant DNA techniques have revolutionized the isolation and production of antibodies. This volume describes methods and technologies which will allow the researcher to isolate a new antibody, analyse its properties, format the correct antibody or fragment, and produce sufficient quantities for experimental use. Topics in this volumes include: generation and analysis of antibodies; antibody repertoires; antibody screening and selection; measuring antibody affinities; sequence analysis; antibody engineering and production; conversion of rodent antibodies to human antibodies by CDR grafting or guided selection; choosing and optimizing effector functions; preparation and use of antibody-based molecules in eukaryotic and prokaryotic systems; scaling up manufacture; and generation of high-affinity high-specificity human antibodies in appropriate formats. Antibody Engineering is a unique manual of recombinant DNA methods for all those working with antibodies in research, diagnosis, and therapeutics. Each chapter is written by a leading researcher in the field and provides essential background information, fully tested protocols, sample data from using these methods, trouble-shooting comments, and key hints and tips for success. This book is intended for post-graduates, post-doctoral researchers, and other academic staff involved in both clinical and molecular biological applications of antibodies.

Full Product Details

Author:   John McCafferty ,  Hennie R. Hoogenboom ,  David J. Chiswell ,  David J. Chiswell (Antibody Technology Limited)
Publisher:   Oxford University Press
Imprint:   Oxford University Press
Volume:   No.169
Dimensions:   Width: 15.00cm , Height: 2.50cm , Length: 23.00cm
Weight:   0.720kg
ISBN:  

9780199635931

ISBN 10:   0199635935
Pages:   325
Publication Date:   01 June 1996
Audience:   College/higher education ,  Professional and scholarly ,  Postgraduate, Research & Scholarly ,  Professional & Vocational
Format:   Hardback
Publisher's Status:   Active
Availability:   To order  
Stock availability from the supplier is unknown. We will order it for you and ship this item to you once it is received by us.

Table of Contents

1: Construction and use of antibody gene repertoires. 2: Affinity maturation of antibodies using phage display. 3: Human antibody repertoires in transgenic mice: manupulation and transfer of YACs. 4: Measuring antibody affinities in solution. 5: Measuring antibody affinities using Bio-sensors. 6: Analysis of human antibody sequences. 7: Rodent to human antibodies by CDR grafting. 8: Rodent to human antibodies by phage display. 9: Choosing and manipulating effector functions. 10: Secretion and purification of antibody fragments expressed in bacteria. 11: Production of single chain Fv monomers and multimers. 12: Expression of immunoglobulin genes in mammalian cells. 13: In vitro linkage of antibody fragments into dimeric and multimeric forms

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