Overview

This second edition provides state-of-the-art and novel methods on antibiotic isolation and purification, identification of antimicrobial killing mechanisms, as well as methods for the analysis and detection of microbial responses and adaptation strategies. Antibiotics: Methods and Protocols,  Second Edition, guides readers through updated and entirely new chapters on production and design, mode of action, and response and resistance. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.  Authoritative and cutting-edge, Antibiotics: Methods and Protocols, Second Edition aims to inspire scientific work in the exciting field of antibiotic research.

Full Product Details

Author:   Peter Sass
Publisher:   Springer-Verlag New York Inc.
Imprint:   Springer-Verlag New York Inc.
Edition:   2nd ed. 2023
Volume:   2601
Weight:   0.983kg
ISBN:  

9781071628546

ISBN 10:   1071628542
Pages:   405
Publication Date:   30 November 2022
Audience:   Professional and scholarly ,  Professional & Vocational
Format:   Hardback
Publisher's Status:   Active
Availability:   Manufactured on demand  
We will order this item for you from a manufactured on demand supplier.

Table of Contents

Part I: Production and Design 1. Antibiotics - precious goods in changing times Peter Sass   2. A Whole-Cell Assay for Detection of Antibacterial Activity in Actinomycetes Culture Supernatants Anika Rütten, Wolfgang Wohlleben, Lena Mitousis, and Ewa Maria Musiol-Kroll   3. Sampling of Human Microbiomes to Screen for Antibiotic-Producing Commensals Benjamin-Torres Salazar, Anna Lange, Laura Camus, and Simon Heilbronner   4. Production of Antimicrobial Compounds by Homologous and Heterologous Expression I Dewa M. Kresna, Zerlina G. Wuisan, and Till F. Schäberle   5. Isolation and Purification of Natural Products from Microbial Cultures Thomas Schafhauser and Andreas Kulik   6. Structure Elucidation of Antibiotics Julia Moschny, Georgios Daletos, Peter Proksch, and Chambers C. Hughes   7. Computer-Aided Drug Design – An update Wenbo Yu,David J. Weber, and Alexander D. MacKerell, Jr.   8. Cytotoxicity Assays as Predictors of the Safety and Efficacy of Antimicrobial Agents Alexander Zipperer, Jasmin Scheurer, and Dorothee Kretschmer   Part II: Mode of Action and Resistance 9. Microscopy-based Multiwell Assay to Characterize Disturbed Bacterial Morphogenesis Upon Antibiotic Action Cruz L. Matos de Opitz and Peter Sass   10. Expansion Microscopy of Bacillus subtilis Viola Middelhauve, Jan-Peter Siebrasse and Ulrich Kubitscheck   11. Tracking global and local changes in membrane fluidity through fluorescence spectroscopy and microscopy Madeleine Humphrey, Ireny Abdelmesseh Nekhala, Kathi Scheinpflug, Oxana Krylova, Ann-Britt Schäfer, Jessica A. Buttress, Michaela Wenzel, and Henrik Strahl   12. Quantitative analysis of microscopy data to evaluate bacterial responses to antibiotic treatment Dominik Brajtenbach, Jan-Samuel Puls, Cruz L. Matos de Opitz, Peter Sass, Ulrich Kubitscheck, and Fabian Grein   13. Application of a Bacillus subtilis whole-cell biosensor (PliaI-lux) for the identification of cell wall active antibacterial compounds Carolin Martina Kobras, Sali May Morris, Thorsten Mascher, and Susanne Gebhard   14. Determination of Bacterial Membrane Impairment by Antimicrobial Agents Miriam Fuerst-Wilmes and Hans-Georg Sahl   15. A colorimetric assay to identify and characterize bacterial primase inhibitors Allan H. Pang and Oleg V. Tsodikov   16. Cell-based fluorescent screen amenable to HTS to Identify Inhibitors of Bacterial Translation Initiation Matteo Raneri, Emilio Alvarez-Ruiz, Danuta Mossakovska, and Federica Briani   17. ­Bacterial two component systems: Overexpression, purification − in vitro and in vivo inhibitor screens Alina Dietrich, Mike Gajdiss, Michael Türck, Ian Monk, and Gabriele Bierbaum   18. Sample preparation for mass-spectrometry based absolute quantification of bacterial proteins in antibiotic stress research Sandra Maaß, Minia Antelo Varela, Florian Bonn, and Dörte Becher   19. Elemental analysis for the characterization of antimicrobial effects Christoph H. R. Senges and Julia E. Bandow   20. label-free quantitation of ribosomal proteins from bacillus subtilis for antibiotic research Sina Schäkermann, Pascal Dietze, and Julia E. Bandow   21. Functional Metagenomics to Study Antibiotic Resistance Bejan Mahmud, Manish Boolchandani, Sanket Patel, and Gautam Dantas

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