Overview

This detailed new edition compiles fully updated practical methodologies and approaches for diagnosticians and researchers working toward a better understanding of animal coronaviruses. The book explores vital techniques in virus isolation, diagnostic approaches via the detection of viral nucleic acids or proteins, serology, disease-targeted animal species models, and next-generation sequencing and bioinformatics. Written for the invaluable Springer Protocols Handbooks series, chapters contain readily reproducible laboratory protocols as well as expert tips on troubleshooting and avoiding known pitfalls.  Thorough and hands-on, Animal Coronaviruses, Second Edition will help readers design and carry out their increasingly important projects in clinical research, diagnostics, disease surveillance, and epidemiological study involving this family of viruses.

Full Product Details

Author:   Leyi Wang
Publisher:   Springer-Verlag New York Inc.
Imprint:   Springer-Verlag New York Inc.
Edition:   2nd ed. 2022
Weight:   0.845kg
ISBN:  

9781071620908

ISBN 10:   1071620908
Pages:   327
Publication Date:   10 February 2022
Audience:   Professional and scholarly ,  Professional & Vocational
Format:   Hardback
Publisher's Status:   Active
Availability:   Manufactured on demand  
We will order this item for you from a manufactured on demand supplier.

Table of Contents

Part I: Reviews   1. Animal Coronaviruses             Leyi Wang and Dongwan Yoo   2. Feline Coronavirus: Insights into the Pathogenesis and Diagnosis             Subarna Barua, Olivia Lockyear, Diane Delmain, and Chengming Wang   3. Animal Models for Studying COVID-19 Pathology and Infection             Kush Kumar Yadav and Scott P. Kenney   Part II: Virus Isolation   4. Isolation of Porcine Epidemic Diarrhea Virus from Clinical Samples             Qiuhong Wang   5. Isolation and Characterization of Porcine Epidemic Diarrhea Virus Using Suspension Vero Cells             Fei-Fei Ge, Hui-Ru Yu, De-Quan Yang, Xin Li, Hou-Bin Ju, Hai-Xiao Shen, Jian Liu, Li-Ping Shen, Hong-jin Zhao, and Jian Wang   6. Isolation and Propagation of Porcine Deltacoronavirus in Cell Culture and Embryonated Chicken Eggs             Hui Hu   7. Isolation and Propagation of Infectious Bronchitis Virus (Avian Coronavirus) in Chicken Embryonated Eggs             Alejandro Banda and Lifang Yan   Part III: Diagnostic Approaches by Detection of Viral Nucleic Acid or Protein   8. Real-Time Reverse Transcription Polymerase Chain Reaction for Simultaneous Detection of Bovine Coronavirus and Other Pathogens             Ailam Lim, Jennifer Godhardt-Cooper, and Kathy Toohey-Kurth   9. TaqMan Real-Time RT-PCR Detection of Porcine Epidemic Diarrhea Virus             Shijuan Dong, Zhen Li, Ruisong Yu, Bingqing Chen, Fusheng Si, and Chunfang Xie   10. Real-Time RT-PCR for Detection of Feline Coronavirus             Leyi Wang, Colleen Olmstead, and Richard Fredrickson   11. SARS-CoV-2 Real-Time RT-PCR Assay in Animals             Leyi Wang, Colleen Olmstead, Karen Terio, and Richard Fredrickson   12. Real-Time PCR and Antigen-Capture ELISA for the Diagnosis of Porcine Deltacoronavirus Infection             Shaopo Zu, Jin Yuan, Xiaohui Jin, and Hui Hu   Part IV: Serology   13. Identification of a Neutralizing Epitope-Containing Segment in Turkey Coronavirus Spike Protein for Diagnostic Serology             Yi-Ning Chen, Ching Ching Wu, and Tsang Long Lin   14. Antibody-Capture Enzyme-Linked Immunosorbent Assay for Detecting Protective Antibodies to Turkey Coronavirus Based on Recombinant Spike Protein Segment with Neutralizing Epitope             Yi-Ning Chen, Mohamed Abdelwahab, Chien Chang Loa, Ming-Kun Hsieh, Ching Ching Wu, and Tsang Long Lin   15. ELISA, Virus Neutralization, and Immunofluorescence Assays for Detection of Antibodies Against Porcine Deltacoronavirus             Lu Xia, Yue Zhang, and Hui Hu   16. Luminescent Immunoprecipitation System (LIPS) Development for the Specific Detection of PDCoV and SARS-CoV-2 Antibodies             Patricia A. Boley and Scott P. Kenney   17. Construction of a Mouse-Feline Chimeric Neutralizing Antibody Against SARS-CoV-2             Fangfeng Yuan, Jin Cui, Xingyu Yan, and Ying Fang   18. Microneutralization Test of Avian Infectious Bronchitis Virus Using Vero Cells             Sheng Zhou, Yi Jiang, and Mengjun Tang   Part V: Disease-Targeted Animal Species as Animal Models   19. Pig Bioassay to Assess Infectivity of Porcine Epidemic Diarrhea Virus Detected in Various Sample Matrices             Jianqiang Zhang   20. Diagnostic Investigation and Confirmation of Emerging Turkey Coronaviral Enteritis             Yi-Ning Chen, Wan-Jung Chen, Ching Ching Wu, and Tsang Long Lin   Part VI: Next-Generation Sequencing and Bioinformatics   21. Whole Genome Sequencing of Porcine Epidemic Diarrhea Virus and Porcine Deltacoronavirus             Ganwu Li, Ying Zheng, Leyi Wang, Yan Zhang, and Huigang Shen   22. Whole-Genome Sequencing of Avian Infectious Bronchitis Virus by iSeq100 Platform and MinION             Lifang Yan, Alejandro Banda, and Leyi Wang   23. Phylogenetic and Recombination Analysis of Animal Coronaviruses             Kai Huang, Huachen Zhu, and Yi Guan

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