Overview

This introductory guide provides novice researchers and lab students with a thorough step-by-step approach to standard animal cell culture techniques. Coverage includes lab safety and best practices, sterility management, preparation, ethical considerations, and troubleshooting for common pain points. This is an up-to-date, indispensable handbook for early-career researchers and students, as well as established scientists in biotechnology, cell and developmental biology, pharmaceutical toxicology, cytogenetics, and more. 

Full Product Details

Author:   Shalini Mani ,  Manisha Singh ,  Anil Kumar
Publisher:   Springer International Publishing AG
Imprint:   Springer International Publishing AG
Edition:   1st ed. 2023
Weight:   0.682kg
ISBN:  

9783031194849

ISBN 10:   3031194845
Pages:   327
Publication Date:   02 February 2023
Audience:   Professional and scholarly ,  Professional & Vocational
Format:   Hardback
Publisher's Status:   Active
Availability:   Manufactured on demand  
We will order this item for you from a manufactured on demand supplier.

Table of Contents

1. Overview to animal cell culture 2. Cell culture laboratory 3. Good lab practices and bio-safety measures 4. Managing sterility in the lab 5. Media and buffer preparation for cell culture 6. Properties of cultured cells and selection of culture media 7. Selection and maintenance of cultured cells 8. Inoculation and passaging of adherent cells 9. Inoculation and passaging of suspension cells 10. Counting of cells 11. Cryopreservation of cell lines 12. Resuscitation of frozen cell line 13. Isolation and culturing of cells from different tissues 14. Differentiation, transformation and immortalization of cells 15. Co-culture techniques 16. 3D cell culture techniques 17. Stem cell culture techniques 18. Identification and removal of biological contamination in the media and cell suspensions 19. Analysis of cell growth kinetics in suspension and adherent types cell line 20. In vitro cytotoxicity analysis: mtt/xtt, trypan blue exclusion 21. Applications of animal cell culture based assays: a. Biomarker Identification b. Genetic Manipulation c. Cellular development and Differentiation d. Pathological studies e. Pharmaceutical studies f. Stem cell Research g. Hybridoma Technology 22. Downstream processing of therapeutically important proteins from cultured cells 23. Ethical issues in animal cell culture 24. Common troubleshooting methods in cell culture techniques

Reviews

Author Information

Dr. Shalini Mani is Associate Professor in the Department of Biotechnology at Jaypee Institute of Information Technology, Noida, India. Dr. Manisha Singh is Assistant Professor and Lecturer in the Department of Biotechnology at Jaypee Institute of Information Technology, Noida, India. Dr. Anil Kumar is Director of Education and Professor at RLB Central Agricultural University, Jhansi, India. 

Tab Content 6

Author Website:  

Customer Reviews

Recent Reviews

No review item found!

Add your own review!

Countries Available

All regions