Overview

This detailed book builds on the foundation of lymphatic protocols with tools to aid in the interpretation of large datasets created by angiogenesis research. For example, this includes protocols to explore how transcriptional phenotyping can be achieved, both in terms of exploring tissue-specific heterogeneity to the use of database mining of existing datasets. Similarly, bioinformatic techniques run alongside protocols for transcript analysis, cell biology, pathology, as well as physiological and developmental angiogenesis models. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.  Authoritative and up-to-date, Angiogenesis: Methods and Protocols provides a meaningful compendium of practical approaches to angiogenesis and endothelial cell biology research.

Full Product Details

Author:   Andrew V. Benest
Publisher:   Springer-Verlag New York Inc.
Imprint:   Springer-Verlag New York Inc.
Edition:   1st ed. 2022
Volume:   2441
Weight:   0.846kg
ISBN:  

9781071620618

ISBN 10:   1071620614
Pages:   429
Publication Date:   02 February 2023
Audience:   Professional and scholarly ,  Professional & Vocational
Format:   Paperback
Publisher's Status:   Active
Availability:   Manufactured on demand  
We will order this item for you from a manufactured on demand supplier.

Table of Contents

Part I: Developmental Biology   1. The Embryonic Mouse Hindbrain Model to Study Sprouting Angiogenesis In Vivo             Gabriela D’Amico and Christiana Ruhrberg   2. A Workflow to Track and Analyze Endothelial Migration during Vascular Development in Zebrafish Embryos Using Lightsheet Microscopy             Yan Chen, Paul C. Evans, and Robert N. Wilkinson   3. Analyzing Vessel Regression and Endothelial Apoptosis as a Component of Angiogenic Vessel Remodeling             Lachlan Whitehead, Emma C. Watson, and Zoe L. Grant   4. Whole-Mount Immunofluorescence Protocol for 3D Imaging, Reconstruction, and Quantification of 4th Pharyngeal Arch Artery Formation             Elena Ioannou and Christiana Ruhrberg   5. Quantifying and Characterizing Angiogenesis Using the Postnatal Mouse Retina             Alessandro Fantin   Part II: Adult Physiology and Pathology   6. Characterization of Lymphatic Vasculature Using Whole-Mount Immunostaining of Mouse Embryonic Dorsal Skin             Nikita Ved and Jacinta Isabelle Kalisch-Smith   7. Analyzing Lymphatic Vessel Patterning in Adult Tissue             Louise A. Johnson   8. Transmission Electron Microscopy of Endothelium             Zubair Ahmed Nizamundeen, Loubna ElSaboni, Grace Vetch, Jumana Karim, Chris R. Neal, Claire L. Allen, David O. Bates, and Kenton P. Arkill   9. Measurement of Revascularisation in the Hind Limb after Experimental Ischemia in Mice             Sohni Ria Bhalla, Federica Riu, Maria J.C. Machado, and David O. Bates   10. Imaging Blood Vessels and Lymphatics in Mouse Trachea Wholemounts             Peter Baluk and Donald M. McDonald   11. Non-Invasive Measurement of Retinal Microvascular Permeability during Loss of Endothelial Quiescence             Naseeb K. Malhi, David O. Bates, Kenton P. Arkill, and Claire L. Allen   12. An Ex Vivo Tissue Culture Method for Discovering Cell Dynamics Involved in Stromal Vascular Fraction Vasculogenesis Using the Mouse Mesentery             Dima Majbour, Ariana D. Suarez-Martinez, Nicholas A. Hodges, Arinola O. Lampejo, Banks M. Lomel, Elijah W. Rice, Hulan Shang, Adam J. Katz, and Walter L. Murfee   13. Quantitative Three-Dimensional Analysis of the Lymphatic Vasculature in the Postnatal Mouse Heart             Konstantinos Klaourakis, Paul R. Riley, and Joaquim Miguel Vieira   14. Quantifying Lymphatic Vessel Density in Human Tissue Samples             Stewart G. Martin, Emad Rakha, and Sarah J. Storr   15. Quantifying Vascular Remodeling in the Mouse Spinal Cord             M.E. Da Vitoria Lobo, Lydia Hardowar, and Richard P. Hulse   16. Quantitative Methods to Assess Adipose Vasculature             Martina Rudnicki, Alexandra Pislaru, and Tara L. Haas   17. Quantification of Angiogenesis in Laser Choroidal Neovascularization             Kathryn R. Green, Nicholas Beazley-Long, Amy P. Lynch, Claire L. Allen, David O. Bates, and Andrew V. Benest   Part III: Reducing Complexity: Growing and Studying Endothelial Cells Outside the Body   18. A Rapid Adaptable Method for Isolation of Endothelial Cells from Human Adipose Tissue             Jacqueline Herold and Joanna Kalucka   19. Simple Gene Knockdown in Endothelial Cells Using Short Interfering RNA Oligonucleotides             Zarah B. Tabrizi, Kathryn R. Green, Amy P. Lynch, Nada S. Ahmed, Nicholas Beazley-Long, and Andrew V. Benest   20. Transcription Factor Chromatin Immunoprecipitation in Endothelial Cells             Philip Kitchen, Kevin Gaston, and Padma-Sheela Jayaraman   21. In Vitro Co-Culture of Fibroblast and Endothelial Cells to Assess Angiogenesis             Adam F. Odell and Aarren J. Mannion   22. Quantification of Leukocyte-Endothelial Cell Interaction during In Vitro Models of Thrombosis at Arterial and Venous Shear Rates             Joshua H. Bourne   23. Isolation of Cardiac Endothelial Cells for Transcriptomic Analysis of the Zebrafish and Mouse Heart             Ziwen Li, Katherine M. Ross Stewart, Finnius A. Bruton, Martin A. Denvir, and Mairi Brittan   24. The Evaluation of Neovessel Angiogenesis Behavior at Tissue Interfaces             Hannah A. Strobel and James B. Hoying   25. Endothelial Cell Fibrin Gel Angiogenesis Bead Assay             Eva Clavane, Hannah Taylor, Richard Cubbon, and Paul Meakin   26. Live Cell Imaging and Analysis of Cancer-Cell Transmigration through Endothelial Monolayers             Aarren J. Mannion   27. Differentiation and Characterization of Human Pluripotent Stem Cell-Derived Cardiac Endothelial Cells for In Vitro Applications             Kavita Raniga, Nguyen T.N. Vo, and Chris Denning   Part IV: Making Use of Publically Existing Datasets and Resources   28. Finding and Verifying Enhancers for Endothelial-Expressed Genes             Alice Neal, Helena Rodriguez-Caro, and Sarah De Val   29. Endothelial Cell RNA-Seq Data: Differential Expression and Functional Enrichment Analyses to Study Phenotypic Switching             Guillermo Díez Pinel, Joseph L. Horder, John R. King, Alan McIntyre, Nigel P. Mongan, Gonzalo Gómez López, and Andrew V. Benest

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