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Author:   J. Goad ,  T. Akihisa
Publisher:   Chapman and Hall
Imprint:   Chapman and Hall
Edition:   1997 ed.
Dimensions:   Width: 15.50cm , Height: 2.50cm , Length: 23.50cm
Weight:   1.810kg
ISBN:  

9780751402308

ISBN 10:   0751402303
Pages:   438
Publication Date:   31 March 1997
Audience:   College/higher education ,  Professional and scholarly ,  Postgraduate, Research & Scholarly ,  Professional & Vocational
Format:   Hardback
Publisher's Status:   Active
Availability:   In Print  
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Table of Contents

1 Nomenclature and biosynthesis of sterols and related compounds.- 1.1 Introduction.- 1.2 IUPAC-IUB recommended nomenclature for steroids and triterpenoids.- 1.3 The biosynthesis of sterols and triterpenes.- 2 Extraction of sterols from tissues.- 2.1 Introduction.- 2.2 Extraction methods.- 2.3 Separation of sterols from extracted lipids.- 2.4 Examples of the extraction of sterols.- References.- 3 Initial separation methods.- 3.1 Introduction.- 3.2 Digitonin precipitation of sterols.- 3.3 Separation of free sterols and steryl conjugates from a total lipid by adsorption column chromatography.- 3.4 Thin-layer chromatography.- 3.5 The use of mini-columns for the preliminary separation of sterol fractions.- 3.6 Hydrolysis of steryl conjugates.- 3.7 Silver nitrate or argentation chromatography.- 3.8 Autoxidation and isomerization of sterols.- References.- 4 High-performance liquid chromatography of sterols.- 4.1 Introduction.- 4.2 Normal- and reversed-phase HPLC.- 4.3 HPLC detectors.- 4.4 HPLC of sterol derivatives.- 4.5 Influence of column temperature on sterol separation by HPLC.- 4.6 Retention data of sterols on HPLC.- 4.7 Sterol separation factors on HPLC.- 4.8 Differentiation of C-24 epimers of 24-alkylsterols on reversed-phase HPLC.- References.- 5 Gas-liquid chromatography of sterols.- 5.1 Introduction.- 5.2 Packed columns and the stationary phase.- 5.3 Capillary columns.- 5.4 Sample injection and detection.- 5.5 Derivatization of sterols.- 5.6 Preparative GC.- 5.7 Retention time data.- 5.8 Separation factors.- 5.9 GC of the C-24 epimers of 24-alkylsterols.- 5.10 GC of steryl conjugates.- References.- 6 Infrared and ultraviolet spectroscopy of sterols.- 6.1 Introduction.- 6.2 Infrared spectroscopy.- 6.3 Ultraviolet spectroscopy.- References.- 7 Mass spectrometry of sterols.- 7.1 Introduction.- 7.2 Sample ionization.- 7.3 Derivatives used for mass spectrometry of sterols.- 7.4 Mass-spectral fragmentations of the sterol rings.- 7.5 Mass-spectral fragmentations of the sterol side chain.- 7.6 Mass spectra of tetracyclic and pentacyclic triterpene alcohols.- 7.7 Mass spectrometry of steryl conjugates.- References.- 8 1H NMR spectroscopy of sterols.- 8.1 Introduction.- 8.2 Methyl signals.- 8.3 Methine and olefinic protons.- 8.4 Quantitative estimation of C-24 epimeric 24-alkylsterol mixtures.- 8.5 Aromatic solvent induced shifts (ASIS).- 8.6 Lanthanide-induced shifts (LIS).- References.- 9 13C NMR spectroscopy of sterols.- 9.1 Introduction.- 9.2 Methods for signal assignments.- 9.3 13C Chemical shift data of sterols and triterpene alcohols.- References.- 10 One-dimensional and two-dimensional NMR spectroscopy of sterols.- 10.1 Introduction.- 10.2 ID NMR spectroscopy.- 10.3 2D NMR spectroscopy.- 10.4 Connectivity diagrams for use with 2D NMR data.- 10.5 Generalized 1D and 2D strategies for sterols and related compounds.- 10.6 Complete assignments of 1H NMR spectra of sterols.- References.- 11 X-ray crystallography of sterols.- References.- 12 Sources of sterols.- 12.1 Introduction.- 12.2 4-Desmethylsterols.- 12.3 Large-scale purification of 4-desmethylsterols.- 12.4 Sterols with methyl groups at C-4 and/or C-14.- 12.5 Tetracyclic triterpene alcohols.- 12.6 Pentacyclic triterpene alcohols and ketones.- 12.7 The use of inhibitors of sterol biosynthesis to produce specific sterols.- 12.8 Sources of sterols and tetra- and pentacyclic triterpenes.- References.- Appendix 1 The trivial names of sterols.- Appendix 2 Structures of pentacyclic triterpenes.- Appendix 3 Physical data of selected sterols and triterpenes.- Appendix 4 The quantification of sterols.

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