Amino Acid Analysis Protocols

Author:   Catherine Cooper ,  Nicole Packer ,  Keith Williams
Publisher:   Humana Press Inc.
Edition:   2000 ed.
Volume:   159
ISBN:  

9780896036567


Pages:   265
Publication Date:   05 September 2000
Format:   Hardback
Availability:   In Print   Availability explained
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Amino Acid Analysis Protocols


Overview

A collection of classic and cutting-edge techniques of high utility in answering specific biological questions about amino acids. Common methods include those based on HPLC or gas chromatography separation and analysis after precolumn derivatization. New techniques based on capillary electrophoresis separation, high-performance anion exchange chromatography, and mass spectrometry are also presented. Each method is described in step-by-step detail to ensure successful experimental results and emphasizes sample preparation, particularly the collection and storage of bodily fluids. Up-to-date and highly practical, Amino Acid Analysis Protocols offers analytical and clinical chemists, as well as a broad range of biological and biomedical investigators, a rich compendium of laboratory tools for the productive analysis of both common and uncommon amino acids.

Full Product Details

Author:   Catherine Cooper ,  Nicole Packer ,  Keith Williams
Publisher:   Humana Press Inc.
Imprint:   Humana Press Inc.
Edition:   2000 ed.
Volume:   159
Dimensions:   Width: 15.50cm , Height: 1.70cm , Length: 23.50cm
Weight:   0.574kg
ISBN:  

9780896036567


ISBN 10:   0896036561
Pages:   265
Publication Date:   05 September 2000
Audience:   College/higher education ,  Professional and scholarly ,  Undergraduate ,  Professional & Vocational
Format:   Hardback
Publisher's Status:   Active
Availability:   In Print   Availability explained
This item will be ordered in for you from one of our suppliers. Upon receipt, we will promptly dispatch it out to you. For in store availability, please contact us.

Table of Contents

Amino Acid Analysis.- Amino Acid Analysis, Using Postcolumn Ninhydrin Detection, in a Biotechnology Laboratory.- Purification of Proteins Using UltraMacro Spin Columns or ProSorb Sample Preparation Cartridges for Amino Acid Analysis.- Amino Acid Analysis Using Precolumn Derivatization with 6-Aminoquinolyl-N-Hydroxysuccinimidyl Carbamate.- Amino Acid Analysis by High-Performance Liquid Chromatography after Derivatization with 1-Fluoro-2,4-Dinitrophenyl-5-L-Alanine Amide (Marfey's Reagent).- The Analysis of Amino Acids Using Precolumn Derivatization, HPLC, and Electrochemical Detection.- Anion Exchange Chromatography and Integrated Amperometric Detection of Amino Acids.- Ion-Pair Chromatography for Identification of Picomolar-Order Protein on a PVDF Membrane.- Capillary Gas Chromatographic Analysis of Protein and Nonprotein Amino Acids in Biological Samples.- Measurement of Blood Plasma Amino Acids in Ultrafiltrates by High-Performance Liquid Chromatography with Automatic Precolumn O-Phthaldialdehyde Derivatization.- Determination of Amino Acids in Foods by Reversed-Phase High-Performance Liquid Chromatography with New Precolumn Derivatives, Butylthiocarbamyl, and Benzylthiocarbamyl Derivatives Compared to the Phenylthiocarbamyl Derivative and Ion Exchange Chromatography.- Amino Acid Measurement in Body Fluids Using PITC Derivatives.- Determination of Proteins, Phosphatidylethanolamine, and Phosphatidylserine in Lipid-Rich Materials by Analysis of Phenylthiocarbamyl Derivatives.- Analysis of O-Phosphoamino Acids in Biological Samples by Gas Chromatography with Flame Photometric Detection.- Determination of Sulfur Amino Acids, Glutathione, and Related Aminothiols in Biological Samples by Gas Chromatography with Flame Photometric Detection.- Capillary Electrophoretic Determination of 4-Hydroxyproline.- Total Plasma Homocysteine Analysis by HPLC with SBD-F Precolumn Derivatization.- Determination of Early Glycation Products by Mass Spectrometry and Quantification of Glycation Mediated Protein Crosslinks by the Incorporation of [14C]lysine into Proteins.

Reviews

Sufficient detail and reference material is provided to allow application by a chemist generally familiar with analytical methods. Choices among the various procedures described will likely be made on an empirical basis. -Doody's Health Sciences Book Review Journal ...the book presents a timely and detailed methodological reference for a broad range of current techniques used in amino acid analysis. The authors are all experts in the field and the editors (especially Williams) have considerable experience in amino acid analysis. this volume will provide an indispensable tool in any laboratory carrying out amino acid analysis. - Today's Life Science ...This is a major collection of analytical techniques devoted to the analysis of amino acids in biotechnology, food analysis, and biomedical research, as well as the amino acid content of patient samples in clinical laboratories...This book is highly recommended for both engineers and experienced analysts interested in amino acid analysis; both will benefit from the information contained in this collection. It might also be a helpful resource for those looking for a replacement for old, dedicated amino acid analyzers for physiological samples by methods based on newer technologies and alternative techniques. - Clinical Chemistry Up-to-date and highly practical, Amino Acid Analysis Protocols offers analytical and clinical chemists as well as a broad range of biological and biomedical investigators a richer compendium of laboratory tools for the productive analysis of both common and uncommon amino acids. - Journal of Chromatographic Science


Sufficient detail and reference material is provided to allow application by a chemist generally familiar with analytical methods. Choices among the various procedures described will likely be made on an empirical basis. - Doody's Health Sciences Book Review Journal the book presents a timely and detailed methodological reference for a broad range of current techniques used in amino acid analysis. The authors are all experts in the field and the editors (especially Williams) have considerable experience in amino acid analysis. this volume will provide an indispensable tool in any laboratory carrying out amino acid analysis. - Today's Life Science This is a major collection of analytical techniques devoted to the analysis of amino acids in biotechnology, food analysis, and biomedical research, as well as the amino acid content of patient samples in clinical laboratories This book is highly recommended for both engineers and experienced analysts interested in amino acid analysis; both will benefit from the information contained in this collection. It might also be a helpful resource for those looking for a replacement for old, dedicated amino acid analyzers for physiological samples by methods based on newer technologies and alternative techniques. - Clinical Chemistry Up-to-date and highly practical, Amino Acid Analysis Protocols offers analytical and clinical chemists as well as a broad range of biological and biomedical investigators a richer compendium of laboratory tools for the productive analysis of both common and uncommon amino acids. - Journal of Chromatographic Science


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