Overview

A panel of highly skilled investigators describe in detail their state-of-the-art biochemical, cell biological, and molecular biological techniques for studying the molecular basis of Alzheimer's disease. These readily reproducible, step-by-step methods focus on work with the amyloid precursor protein and the amyloidogenic peptide As, but also include - with the recent identification of presenilin proteins - techniques for determining the structure and biological function of these proteins. In addition, there are chapters covering the tau protein and its role in Alzheimer's disease, as well as an introductory discussion of the history of the disease, its genetic basis, and the currently available and possible future therapeutic agents. Cutting-edge and wide ranging, Alzheimer's Disease: Methods and Protocols provides ready access to proven, reproducible methods for elucidating the molecular basis of this most common senile dementia.

Full Product Details

Author:   Nigel M. Hooper
Publisher:   Humana Press Inc.
Imprint:   Humana Press Inc.
Edition:   2000 ed.
Volume:   32
Dimensions:   Width: 15.50cm , Height: 2.60cm , Length: 23.50cm
Weight:   1.760kg
ISBN:  

9780896037373

ISBN 10:   0896037371
Pages:   408
Publication Date:   29 December 1999
Audience:   College/higher education ,  Professional and scholarly ,  Undergraduate ,  Professional & Vocational
Format:   Hardback
Publisher's Status:   Active
Availability:   In Print  
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Table of Contents

to Alzheimer's Disease.- The Genetics of Alzheimer's Disease.- Advances in Methodology and Current Prospects for Primary Drug Therapies for Alzheimer's Disease.- Production and Functional Assays of Recombinant Secreted Amyloid Precursor Protein (APP) (sAPP?).- Quantifying A?1–40 and A?1–42 Using Sandwich-ELISA.- Electrophoretic Separation and Immunoblotting of A?1–40 and A?1–42.- A?-Induced Proinflammatory Cytokine Release from Differentiated Human THP-1 Monocytes.- Effects of the ?-Amyloid Peptide on Membrane Ion Permeability.- Analysis of ?-Amyloid Peptide Degradation In Vitro.- Posttranslational Modifications of Amyloid Precursor Protein.- Posttranslational Modifications of the Amyloid Precursor Protein.- Using an Amyloid Precursor Protein (APP) Reporter to Characterize ?-Secretase.- Inhibition of ?-Secretase by Zinc Metalloproteinase Inhibitors.- Development of Neoepitope Antibodies Against the ?-Secretase Cleavage Site in the Amyloid Precursor Protein.- ?-Secretase.- Using ?-Secretase Inhibitors to Distiguish the Generation of the A? Peptides Terminating at Val-40 and Ala-42.- Designing Animal Models of Alzheimer's Disease with Amyloid Precursor Protein (APP) Transgenes.- Phosphorylation of Amyloid Precursor Protein (APP) Family Proteins.- Determining the Transmembrane Topology of the Presenilins.- Normal Proteolytic Processing of the Presenilins.- Apoptotic Proteolytic Cleavage of the Presenilins by Caspases.- The Phosphorylation of Presenilin Proteins.- Interaction of the Presenilins with the Amyloid Precursor Protein (APP).- Distribution of Presenilins and Amyloid Precursor Protein (APP) in Detergent-Insoluble Membrane Domains.- Characterization and Use of Monoclonal Antibodies to Tau and Paired Helical Filament Tau.- Tau PhosphorylationBoth In Vitro and in Cells.- Transglutaminase-Catalyzed Formation of Alzheimer-Like Insoluble Complexes from Recombinant Tau.

Reviews

...this volume contains well-written chapters containing factual insights and useful clarifications. An example is David Allsop's introduction of the field through the orthodox views of the amyloid cascade hypothesis that in the exposition of others is often quite simplistic, but here is presented with complexity and wisdom deserving of serious consideration....they are provided in a format that invites the reader to undertake studies and examine the underpinnings of the subject beyond that found in the primary publications and not covered in such a practical manner in other reviews. - Journal of Molecular Neuroscience

...this volume contains well-written chapters containing factual insights and useful clarifications. An example is David Allsop's introduction of the field through the orthodox views of the amyloid cascade hypothesis that in the exposition of others is often quite simplistic, but here is presented with complexity and wisdom deserving of serious consideration...they are provided in a format that invites the reader to undertake studies and examine the underpinnings of the subject beyond that found in the primary publications and not covered in such a practical manner in other reviews. - Journal of Molecular Neuroscience

this volume contains well-written chapters containing factual insights and useful clarifications. An example is David Allsop's introduction of the field through the orthodox views of the amyloid cascade hypothesis that in the exposition of others is often quite simplistic, but here is presented with complexity and wisdom deserving of serious consideration. they are provided in a format that invites the reader to undertake studies and examine the underpinnings of the subject beyond that found in the primary publications and not covered in such a practical manner in other reviews. - Journal of Molecular Neuroscience

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