Overview

This detailed volume collects commonly used and cutting-edge methods to analyze alternative splicing, a key step in gene regulation. After an introduction of the alternative splicing mechanism and its targeting for therapeutic strategies, the book continues with techniques for analyzing alternative splicing profiles in complex biological systems, visualizing and localizing alternative spliced transcripts with cellular and sub-cellular resolution, probing regulators of alternative splicing, as well as assessing the functional consequences of alternative splicing. Written for the highly successful Methods in Molecular Biology series, chapters include introduction to their respective topics, lists of the necessary materials and reagents, step-by-step, reproducible protocols, and tips on troubleshooting and avoiding known pitfalls.  Authoritative and practical, Alternative Splicing: Methods and Protocols serves as an ideal guide for both RNA aficionados that want to implement novel approaches in their labs and novices undertaking alternative splicing projects.

Full Product Details

Author:   Peter Scheiffele ,  Oriane Mauger
Publisher:   Springer-Verlag New York Inc.
Imprint:   Springer-Verlag New York Inc.
Edition:   1st ed. 2022
Volume:   2537
Weight:   0.889kg
ISBN:  

9781071625200

ISBN 10:   1071625209
Pages:   354
Publication Date:   28 July 2022
Audience:   Professional and scholarly ,  Professional & Vocational
Format:   Hardback
Publisher's Status:   Active
Availability:   Manufactured on demand  
We will order this item for you from a manufactured on demand supplier.

Table of Contents

1. Alternative Splicing in Human Biology and Disease             Daniel Jutzi and Marc-David Ruepp   2. Targeting Alternative Splicing for Therapeutic Interventions             Jessica L. Centa and Michelle L. Hastings   3. An Optimized Protocol for the Mapping of Cell Type-Specific Ribosome-Associated Transcript Isoforms from Small Mouse Brain Regions             Giulia Di Bartolomei and Peter Scheiffele   4. FACS-Based Neuronal Cell Type-Specific RNA Isolation and Alternative Splicing Analysis             Zicheng Wei, Yuxin Qin, Gordon Fishell, and Boxing Li   5. Quantitative Measurement of Alternatively Spliced RNA Isoform Levels             Zhelin Jeff Li and Sika Zheng   6. Analysis of Splicing Regulation by Third-Generation Sequencing             Eric Allemand and Fabrice Ango   7. Computational Analysis of Alternative Splicing Using VAST-TOOLS and the VastDB Framework             André Gohr, Federica Mantica, Antonio Hermoso-Pulido, Javier Tapial, Yamile Márquez, and Manuel Irimia   8. Identification and Quantification of Microexons Using Bulk and Single-Cell RNA-Seq Data             Guillermo E. Parada and Martin Hemberg   9. Functional Annotation of Custom Transcriptomes             Fursham Hamid, Kaur Alasoo, Jaak Vilo, and Eugene Makeyev   10. Design, Labeling, and Application of Probes for RNA smFISH             Ewa Piskadlo, Bastian Th. Eichenberger, Luca Giorgetti, and Jeffrey A. Chao   11. BaseScope™ Approach to Visualize Alternative Splice Variants in Tissue             Alexandra Bunda and Arturo Andrade   12. In Situ Imaging of mRNA Splicing Variants by SpliceRCA             Xiaojun Ren and Jinghong Li   13. Two-Color Fluorescent Reporters for Analysis of Alternative Splicing             John A. Calarco and Pallavi P. Pilaka   14. Quantitative Detection of Protein Splice Variants by Selected Reaction Monitoring (SRM) Mass Spectrometry             Alexander Schmidt and Dietmar Schreiner   15. Probing the Interactions of Splicing Regulatory Small Molecules and Proteins with U1 snRNP Using NMR Spectroscopy             Sébastien Campagne, Tebbe de Vries, and Frédéric H-T. Allain   16. Genome-Wide CRISPR Screening to Identify Mammalian Factors that Regulate Intron Retention             Anna M. Scarborough, Ashwin Govindan, and Nicholas K. Conrad   17. Tethered Function Assays to Elucidate the Role of RNA-Binding Proteins             Andrea B. Eberle and Oliver Mühlemann   18. Probing Liquid-Liquid Phase Separation of RNA-Binding Proteins In Vitro and In Vivo             Stephanie Heinrich and Maria Hondele   19. Analysis of Oligonucleotide Biodistribution and Metabolization in Experimental Animals             François Halloy, Paulina Ćwiek, Jonathan Hall, and Daniel Schümperli

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