Overview

The second edition of Agrobacterium Protocols contains 80 chapters (two volumes) divided into 14 parts. In Volume I, there are a total of six parts. Part I (Agrobacterium handling) provides six chapters describing basic techniques in Agrobacterium manipulation and strategies for vector construction, major components of plant transformation that are often neglected by many plant biologists. Part II (Model plants) consists of seven chapters describing various ways to introduce DNA into three major model plant species, Arabidopsis thaliana, Medicago truncatula, and Nicotiana. Although most plant laboratories transform these model plants on a routine basis, protocols from leading experts may further enhance their capabilities. Parts III through XIII collect 61 chapters covering protocols for 59 plant species. The plants are grouped according to their practical utilization rather than their botanical classification. The significant expansion of this section reflects the remarkable advancements in plant transformation technology during the past decade. Volume I contains four of the eleven parts (Part III to VI) of plant protocols.; This book provides a bench-top manual for tested protocols involving Agrobacterium-mediated transformation.

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9781280832307

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