Overview

This volume details Agrobacterium strain methods, such as  plant genotype, transient and stable transformation of plants for gene overexpression, silencing, transplastomic and editing. Transformation protocols are focused mainly in plants with economic importance such as cereals and fruit trees including diploids and polyploids, and in non-plant species such fungi and algae. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and key tips on troubleshooting and avoiding known pitfalls.   Authoritative and cutting-edge, Agrobacterium: Methods and Protocols aims to be a useful and practical guide to new researchers and experts looking to expand their knowledge. 

Full Product Details

Author:   Claudia Stange Klein
Publisher:   Springer-Verlag New York Inc.
Imprint:   Springer-Verlag New York Inc.
Volume:   2911
ISBN:  

9781071644492

ISBN 10:   1071644491
Pages:   200
Publication Date:   28 March 2025
Audience:   College/higher education ,  Professional and scholarly ,  Postgraduate, Research & Scholarly ,  Professional & Vocational
Format:   Hardback
Publisher's Status:   Active
Availability:   Manufactured on demand  
We will order this item for you from a manufactured on demand supplier.

Table of Contents

Preparation of chemocompetent Agrobacterium tumefaciens cells.- Molecular analysis of Agrobacterium tumefaciens transformed strains with commercially available Plasmid DNA Miniprep kits.- Using Agrobacterium tumefaciens to assemble multi-step metabolic pathways in Nicotiana benthamiana.- Verifying plasmid constructs via transient Agrobacterium tumefaciens-mediated plant transformation in Nicotiana benthamiana.- Vacuum-Assisted Transient Agrobacterium tumefaciens-Mediated Transformation for plant molecular analysis.- Agrobacterium tumefaciens-mediated plant transformation and gene editing in rice.- Agrobacterium-Mediated Transformation for Commercial Wheat Varieties.- Agrobacterium-mediated transformation of Soybean (Glycine max L.) using split-cotyledonary explant.- Genetic transformation of Lettuce (Lactuca sativa) using Agrobacterium tumefaciens.- Agrobacterium tumefaciens-mediated genetic transformation of Nigella damascene.- Agrobacterium tumefaciens mediated stable cotyledon transformation of Solanum lycopersicum var Micro-Tom.- Agrobacterium-mediated transformation for gene editing tomato elite breeding lines.- Agrobacterium tumefaciens-mediated stable transformation of Malus domestica (Apple) cv Fuji RakuRaku and Royal Gala.- Agrobacterium tumefaciens-Mediated Transformation of Grape (Vitis vinífera sp).- Agrobacterium-Mediated Stable Transformation of Actinidia deliciosa (Kiwi).- Transformation of mature apricot (Prunus armeniaca L.) hypocotyl slices.- Agrobacterium-Mediated Transformation of Prunus persica (Peach) Immature Cotyledons.

Reviews

Author Information

Tab Content 6

Author Website:  

Countries Available

All regions