Overview

This volume provides a compilation of methods that will aid researchers studying virology, immunology, and vaccinology of this devastating disease of swine. Written in the format of the highly successful Methods in Molecular Biology series, each chapter includes an introduction to the topic, lists necessary materials and reagents, includes tips on troubleshooting and known pitfalls, and step-by-step, readily reproducible protocols. Authoritative and cutting-edge, African Swine Fever Virus: Methods and Protocols aims to be a foundation for future studies and to be a source of inspiration for new investigations in the field.  

Full Product Details

Author:   Christopher L. Netherton
Publisher:   Springer-Verlag New York Inc.
Imprint:   Springer-Verlag New York Inc.
Edition:   1st ed. 2022
Volume:   2503
Weight:   0.642kg
ISBN:  

9781071623329

ISBN 10:   107162332
Pages:   218
Publication Date:   17 May 2022
Audience:   Professional and scholarly ,  Professional & Vocational
Format:   Hardback
Publisher's Status:   Active
Availability:   Manufactured on demand  
We will order this item for you from a manufactured on demand supplier.

Table of Contents

1               Working safely with African swine fever virus. Sarah Gold, Anna Lawton, and Graeme Harkess   2               Necropsy procedures and evaluation of macroscopic lesions of pigs infected with African swine fever virus. Pedro J. Sánchez-Cordón, Fabian Lean, Matthieu Bernard, and Alejandro Núñez   3               Preparation of immunofluorescently labelled tissue sections for imaging at low and high magnifications in the confocal microscope. Jennifer Simpson and Pippa Hawes   4               Primary macrophage culture from porcine blood and lungs. Lynnette C. Goatley, Rachel Nash, and Christopher L Netherton   5               Isolation of porcine bone marrow cells and generation of recombinant African swine fever viruses. Anusyah Rathakrishnan, Ana Luisa Reis, Katy Moffat, and Linda K. Dixon   6               Laboratory diagnosis and quantification of African swine fever virus using real-time polymerase chain reaction. Christopher L Netherton, Lynnette C Goatley, John Flannery, Martin Ashby, and Carrie Batten   7               Quantification of ASFV DNA and RNA in Ornithodoros soft ticks Rémi Pereira De Oliveira, Laurence Vial, and Marie-Frédérique Le Potier   8               Genotyping of African swine fever virus. Paulina Rajko-Nenow and Carrie Batten   9               African swine fever virus (ASFV) indirect ELISA test based on the use of the soluble cytoplasmic semi purified antigen (ASFV CP-Ag). Carmina Gallardo, Raquel Nieto, and Marisa Arias   10            Indirect immunoperoxidase test (IPT) for detection of antibodies against African swine fever virus (ASFV) on African green monkey cell lines (Vero, MS) Carmina Gallardo, Raquel Nieto, and Marisa Arias   11            African Swine Fever Virus Haemadosrption Inhibition Assay Alexander Malogolovkin and Alexey Sereda   12            ELISpot assay for the detection of ASFV-specific interferon-gamma (IFN-γ) producing cells Raquel Portugal   13            Purification of African swine fever virus. Gareth L. Shimmon, Pranav NM Shah, Elizabeth Fry, David I Stuart, Pippa Hawes and Christopher L. Netherton   14            African swine fever virus plaque assay and disinfectant testing. Lorraine Frost and Carrie Batten   15            Bioorthogonal labelling of African swine fever virus infected cells. Sophie-Marie Aicher   16            Whole genome sequencing of African swine fever virus. Ha Thi Thanh Tran, Anh Duc Truong, and Hoang Vu Dang

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