Overview

Forty years after the term “affinity chromatography” was introduced, this mode of chromatography remains a key tool in the armory of separation techniques that are available to separation and interaction scientists. Affinity chromat- raphy is favored because of its high selectivity, speed, and ease of use. The rapid and selective isolation of molecules using affinity chromatography has allowed a better understanding of biological processes, accelerated the identi- cation of target molecules, and spawned new process areas such as immobilized enzyme reactors. It has had ubiquitous application in most areas of science ranging from small molecule isolation to biopolymers such as DNA, proteins, polysaccharides, and even whole cells. The number of applications of affinity chromatography continues to expand at a rapid rate. For example, more than 60% of purification protocols include some sort of affinity chromatography step, while a database search of PubMed reveals more than 36,000 publications making use of the term “affinity chromatography,” more than 3000 of which refer to it in their title. The US patent office reports more than 16,000 ref- ences to the term “affinity chromatography”, while there are more than 270 references to the same term in the patent title. The aim of this edition of Methods in Molecular Biology, Affinity Chromatography: Methods and Protocols, Second Edition is to provide the beginner with the practical knowledge to develop affinity separations suitable for various applications relevant to the post-genomic era.

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9781617377013

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Reviews

From the reviews of the second edition: The book consists of three parts including 21 chapters that have been written by a large group of authors from many countries. ! recommends this book as a manual for many specialists involved in purification of biomolecules in various fields of biochemistry, molecular biology, biotechnology, and bioorganic chemistry. The book can also be recommended as a handbook for university students and their teachers as well as for other schools preparing specialists in the above-mentioned fields. (G. Ya. Wiederschain, Biochemistry, November, 2008)

From the reviews of the second edition: The book consists of three parts including 21 chapters that have been written by a large group of authors from many countries. ... recommends this book as a manual for many specialists involved in purification of biomolecules in various fields of biochemistry, molecular biology, biotechnology, and bioorganic chemistry. The book can also be recommended as a handbook for university students and their teachers as well as for other schools preparing specialists in the above-mentioned fields. (G. Ya. Wiederschain, Biochemistry, November, 2008)

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