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Author:   Toshiya Senda ,  Katsumi Maenaka
Publisher:   Springer Verlag, Japan
Imprint:   Springer Verlag, Japan
Edition:   1st ed. 2016
Dimensions:   Width: 17.80cm , Height: 2.40cm , Length: 25.40cm
Weight:   8.842kg
ISBN:  

9784431560289

ISBN 10:   4431560289
Pages:   340
Publication Date:   03 August 2016
Audience:   College/higher education ,  Postgraduate, Research & Scholarly
Format:   Hardback
Publisher's Status:   Active
Availability:   Manufactured on demand  
We will order this item for you from a manufactured on demand supplier.

Table of Contents

Part 1. Protein expression.- 1 Expression in Bacteria and Refolding.- 2 Expression of proteins in insect and mammalian cells.- 3 Application of MultiBac system to large complexes.- 4 Purification using affinity-tag technology.- 5 Cell-Free Protein Production for Structural Biology.- Part 2. Purification and crystallization of membrane proteins.- 6 Overview of membrane protein purification and crystallization.- 7 Cell-free synthesis of membrane proteins.- Part 3. Crystallization and Crystal Engineering.- 8 Screening of cryoprotectants and the multistep soaking method.- 9 Protein modification for crystallization.- Part 4. Interaction analysis.- 10 Analytical ultracentrifugation.- 11 Mass spectrometry.- 12 Frontal gel filtration.- 13 Surface Plasmon Resonance.- Part 5. Advanced methods for structural analyses.- 14 Structural biology with micro-focus beam-lines.- 15 Structural biology and electron microscopy.- 16 Structure determination software for macromolecular X-ray crystallography.- 17 NMR Structural Biology using Paramagnetic Lanthanide Probe.

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