Overview

Since the publication of the first edition, the techniques and experimental approaches employed in the study of adipose tissue have undergone radical expansion and revision. In Adipose Tissue Protocols: Second Edition, several updated chapters are combined with many new contributions from internationally recognized experts covering both conventional and state-of-the-art methodologies as well as whole animal, cellular and molecular approaches which have emerged in or been extended to adipose tissue research over the past few years. As a volume in the highly successful Methods in Molecular Biology™ series, each chapter includes an introduction to its subject matter, a list of necessary materials, step-by-step, readily reproducible laboratory protocols, and a Notes section, which provides invaluable tips and tricks to safeguard against potential mishaps and pitfalls. Cutting-edge and comprehensive, Adipose Tissue Protocols: Second Edition is an essential manual for newcomers tothis burgeoning area as well as a valuable addition to the laboratories and offices of more experienced researchers.

Full Product Details

Author:   Kaiping Yang
Publisher:   Humana Press Inc.
Imprint:   Humana Press Inc.
Edition:   Softcover reprint of hardcover 2nd ed. 2008
Volume:   456
Dimensions:   Width: 15.50cm , Height: 2.00cm , Length: 23.50cm
Weight:   0.598kg
ISBN:  

9781617378447

ISBN 10:   1617378445
Pages:   335
Publication Date:   01 December 2010
Audience:   Professional and scholarly ,  Professional & Vocational
Format:   Paperback
Publisher's Status:   Active
Availability:   Manufactured on demand  
We will order this item for you from a manufactured on demand supplier.

Table of Contents

Overview of Adipose Tissue and Its Role in Obesity and Metabolic Disorders.- Choosing an Adipose Tissue Depot for Sampling.- Application of Imaging and Other Noninvasive Techniques in Determining Adipose Tissue Mass.- Generation of Adipose Tissue-Specific Transgenic Mouse Models.- Angiogenesis in Adipose Tissue.- Adipose Organ Nerves Revealed by Immunohistochemistry#.- Human Adipose Tissue Blood Flow and Micromanipulation of Human Subcutaneous Blood Flow.- Studies of Thermogenesis and Mitochondrial Function in Adipose Tissues.- Application of Lipidomics and Metabolomics to the Study of Adipose Tissue.- Applications of Proteomics to the Study of Adipose Tissue.- Application of DNA Microarray to the Study of Human Adipose Tissue/Cells.- Differentiation of Adipose Stem Cells.- Methods That Resolve Different Contributions of Clonal Expansion to Adipogenesis in 3T3-L1 and C3H10T1/2 Cells.- Explant Cultures of White Adipose Tissue.- Isolation and Culture of Preadipocytes from Rodent White Adipose Tissue.- Flow Cytometry on the Stromal-Vascular Fraction of White Adipose Tissue.- Application of Electrophoretic Mobility Shift Assay and Chromatin Immunoprecipitation in the Study of Transcription in Adipose Cells.- Application of RNA Interference Techniques to Adipose Cell Cultures.- RNA Isolation and Real-Time Quantitative RT-PCR.- Study of Adipose Tissue Gene Expression by In Situ Hybridization.- Application of Immunocytochemistry and Immunofluorescence Techniques to Adipose Tissue and Cell Cultures.- Determination of Lipolysis in Isolated Primary Adipocytes.- Study of Glucose Uptake in Adipose Cells.- Measurement of Phosphoinositide 3-Kinase and Its Products to Study Adipogenic Signal Transduction.

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