Overview

At present where protein identification and characterisation using mass spectrometry is a method of choice, this book is presenting a review of basic proteomic techniques. The second part of the book is related to the novel high throughput protein identification technique called the 'molecular scanner'.

Several protein identification techniques are described, especially the peptide mass fingerprint with MALDI-MS based method. E.g. ionisation process, matrix available, signal reproducibility and suppression effect, as well as date treatment for protein identification using bioinformatics tools.

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9789048102563

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<p> This book is timely. It reviews in a concise form most techniques that should be known by scientists working in a proteomics laboratory or analysing proteins of interest. It first reviews the electrophoretic and chromatographic separation methods. It then summarizes the quantification and identification methods such as immuno-blotting, protein chemistry, peptide fingerprinting or sequencing by fragmentation. Several chapters highlight fascinating developments in the field of mass spectrometry and related techniques. The text shows the reader the perspective of this relatively new field of proteomics. <br>Finally, the book lists numerous references to critical work done many years ago and unavailable on computer databases. It should therefore be part of every laboratory s library. <br>(Professor D.F. Hochstrasser)

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