Overview

The critically acclaimed laboratory standard for more than forty years, Methods in Enzymology is one of the most highly respected publications in the field of biochemistry. Since 1955, each volume has been eagerly awaited, frequently consulted, and praised by researchers and reviewers alike. More than 285 volumes have been published (all of them still in print) and much of the material is relevant even today-truly an essential publication for researchersin all fields of life sciences.

Full Product Details

Author:   John N. Abelson (California Institute of Technology, Division of Biology, Pasadena, U.S.A.) ,  Melvin I. Simon (The Salk Institute, La Jolla, CA, USA) ,  Suresh V. Ambudkar (National Cancer Institute, National Institutes of Health, Bethesda, Maryland) ,  Michael M. Gottesman (National Cancer Institute, National Institutes of Health, Bethesda, Maryland, U.S.A.)
Publisher:   Elsevier Science Publishing Co Inc
Imprint:   Academic Press Inc
Volume:   v. 292
Weight:   1.360kg
ISBN:  

9780121821937

ISBN 10:   0121821935
Pages:   853
Publication Date:   27 August 1998
Audience:   Professional and scholarly ,  Professional & Vocational
Format:   Hardback
Publisher's Status:   Active
Availability:   Out of stock  
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Table of Contents

Prokaryotic ABC Transporters:Overview of Bacterial ABC TransportersPreparation and Reconstitution of Membrane-Associated Maltose Transporter Complex of Escherichia coli.Maltose Transport in Escherichia coli: Mutations That Uncouple ATP Hydrolysis from TransportBinding Protein-Dependent ABC Transport System for Glycerol 3-Phosphate of Escherichia coli. Eukaryotic ABC Transporters:Cloning of Novel ABC Transporter GenesABC Proteins and Their Relevance to Human Health and Disease. A. Nonmammalian ABC Transport Systems:MDR Genes: Molecular Cloning and Protein Chemical AspectsFunctional Analysis of pfmdr1 Gene of Plasmodium falciparumAmplification of ABC Transporter Gene pgpA and of Other Heavy Metal Resistance Genes in Leishmania tarentolae and Their Study by Gene Transfection and Gene DisruptionFunctional Assay for Analysis of Yeast ste6 MutantsABC Transporters Involved in Transport of Eye Pigment Precursors in Drosophila melanogaster. B. Mammalian P-Glycoproteins:Isolation of Altered Function Mutants and Genetic Supressor Elements of Multidrug Transporter P-Glycoprotein by Expression Selection from Retroviral LibrariesSelection and Maintenance of Multidrug-Resistant CellsMonoclonal Antibodies to P-Glycoprotein: Preparation and Applications to Basic and Clinical ResearchTopology of P-GlycoproteinsUse of Cell-Free System to Determine P-Glycoprotein Transmembrane TopologyPhotoaffinity Labels for Characterizing Drug Interaction Sites of P-GlycoproteinIdentification of Drug Interaction Sites in P-GlycoproteinPhotoaffinity Labeling of Human P-Glycoprotein: Effect of Modular Interaction and ATP Hydrolysis on Substrate BindingIdentification of Phosphorylation Sites in Human MDR1 P-GlycoproteinIdentification of In Vivo Phosphorylation Sites for Basic-Directed Kinases in Murine mdr1b P-Glycoprotein by Combination of Mass Spectrometry and Site-Directed MutagenesisP-Glycoprotein and Swelling-Activated Chloride ChannelsFunctional Expression of mdr and mdr-like cDNAs in Escherichia coliYeast Secretory Vesicle System for Expression and Functional Characterization of P-GlycoproteinsHigh Level Expression of Mouse Mdr3 P-Glycoprotein in Yeast Pichia pastoris and Characterization of ATPase ActivityMutational Analysis of P-Glycoprotein in Yeast Saccaromyces cerevisiaeBaculovirus-Mediated Expression of Human Multidrug Resistance cDNA in Insect Cells and Functional Analysis of Recombinant P-GlycoproteinRecombinant Vaccinia Virus Vectors for Functional Expression of P-Glycoprotein in Mammalian CellsFunctional Expression of Human P-Glycoprotein from Plasmids Using a Vaccinia Virus-Bacteriophage T7 RNA Polymerase SystemA pHaMDR-DHFR Bicistronic Expression System for Mutational Analysis of P-GlycoproteinMutational Analysis of Human P-GlycoproteinPurification and Reconstruction of Human P-GlycoproteinDrug-Stimulatable ATPase Activity in Crude Membranes of Human MDR1-Transfected Mammalian CellsATPase Activity of Chinese Hamster P-GlycoproteinConstruction of MDR1 Vectors for Gene TherapyConstruction of MDR1 Adeno-Associated Virus for Gene TherapyRetroviral Transfer of the Human MDR 1 into Human T-LymphocytesConstruction and Analysis of Multidrug Resistance Transgenic Mice. C. Multidrug Resistance Associated Protein:Cloning, Transfer, and Characterization of Multidrug Resistance ProteinTransport Function and Substrate Specificity of Multidrug Resistance Protein. D. Cystic Fibrosis Transmembrane Conductance Regulator:Heterologous Expression Systems for Study of Cystic Fibrosis Transmembrane Conductance RegulatorCharacterization of Polyclonal and Monoclonal Antibodies to Cystic Fibrosis Transmembrane Conductance RegulatorIdentification of Cystic Fibrosis Transmembrane Conductance Regulator in Renal EndosomesAssays of Dynamics, Mechanisms, and Regulation of ATP Transport and Release: Implications for Study of ABC Transporter FunctionOverexpression, Purification, and Function of First Nucleotide Binding Fold of Cystic Fibrosis Transmembrane Conductance RegulatorRecombinant Synthesis of Cystic Fibrosis Transmembrane Conductance Regulator and Functional Nucleotide Binding DomainsCationic Lipid Formulations for Intracellular Gene Delivery of Cystic Fibrosis Transmembrane Conductance Regulator to Airway EpitheliaAdeno-Associated Virus Vectors for Gene Therapy of Cystic Fibrosis. E. Sulfonylurea Receptor:Sulfonylurea Receptors and ATP-Sensitive Potassium Ion Channels F. Intracellular ABC Transporters:Peptide Transport Assay for TAP FunctionPeroxisomal ABC TransportersMitochondrial ABC Transporters

Reviews

Praise for the Volume ...the book describes techniques so diverse that they include all major laboratory approaches of modem biochemistry, cell biology, and molecular biology. It provides step-by-step protocols and overviews in the field that also allow newcomers to obtain an orientation over a wide spectrum of aspects... The content is very carefully edited for clarity and precision of the described procedures. Anyone in possession of the equipment needed will be able to reproduce the described conditions and methods. --DOODY'S PUBLISHING REVIEWS Praise for the Series The Methods in Enzymology series represents the gold-standard. --NEUROSCIENCE Incomparably useful. --ANALYTICAL BIOCHEMISTRY It is a true 'methods' series, including almost every detail from basic theory to sources of equipment and reagents, with timely documentation provided on each page. --BIO/TECHNOLOGY The series has been following the growing, changing and creation of new areas of science. It should be on the shelves of all libraries in the world as a whole collection. --CHEMISTRY IN INDUSTRY The appearance of another volume in that excellent series, Methods in Enzymology, is always a cause for appreciation for those who wish to successfully carry out a particular technique or prepare an enzyme or metabolic intermediate without the tiresome prospect of searching through unfamiliar literature and perhaps selecting an unproven method which is not easily reproduced. --AMERICAN SOCIETY OF MICROBIOLOGY NEWS If we had some way to find the work most often consulted in the laboratory, it could well be the multi-volume series Methods in Enzymology...a great work. --ENZYMOLOGIA A series that has established itself as a definitive reference for biochemists. --JOURNAL OF CHROMATOGRAPHY

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