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Author:   Leslie Wilson (University of California, Santa Barbara, USA) ,  Paul T. Matsudaira (Whitehead Institute for Biomedical Research and Massachusetts Institute of Technology, Cambridge, U.S.A.) ,  Richard Nuccitelli (Division of Biological Sciences, University of California, Davis, U.S.A.)
Publisher:   Elsevier Science Publishing Co Inc
Imprint:   Academic Press Inc
Volume:   v. 40
Dimensions:   Width: 15.20cm , Height: 2.30cm , Length: 22.90cm
Weight:   0.780kg
ISBN:  

9780125228107

ISBN 10:   0125228104
Pages:   368
Publication Date:   18 May 1994
Audience:   Professional and scholarly ,  Professional & Vocational
Format:   Paperback
Publisher's Status:   Active
Availability:   Manufactured on demand  
We will order this item for you from a manufactured on demand supplier.

Table of Contents

Ca2+ Buffers and [Ca2+]I Perturbation Techniques: D.M. Bers, C.W. Patton, and R. Nuccitelli, A Practical Guide to the Preparation of Ca2+ Buffers. R. Zucker, Photorelease Techniques for Raising or Lowering Intracellular Ca2+. D. Thomas and M.R. Hanley, Pharmacological Tools for Perturbing Intracellular Calcium Storage. Microelectrode Techniques for Measuring [Ca2+]I and Ca2+ Fluxes: S. Baudet, L. Hove-Madsen, and D.M. Bers, How to Make and to Use Calcium-Specific Mini- and Microelectrodes. P.J.S. Smith, R.H. Sanger, and L.F. Jaffe, The Vibrating Ca2+ Electrode: A New Technique for Detecting Plasma Membrane Regions of Ca2+ Influx and Efflux. C.A. Leech and G.G. Holz, IV, Application of Patch Clamp Methods to the Study of Calcium Currents and Calcium Channels. Fluorescence Techniques for Imaging [Ca2+]I: J.P.Y. Kao, Practical Aspects of Measuring [Ca2+] with Fluorescent Indicators. S.J. Morris, T.B. Wiegmann, L.W. Welling, and B.M. Chronwall, Rapid Simultaneous Estimation of Intracellular Calcium and pH. T.J. Keating and R.J. Cork, Improved Spatial Resolution in Ratio Images Using Computational Confocal Techniques. P.A. Diliberto, X.F. Wang, and B. Herman, Confocal Imaging of Ca2+ in Cells. S. Girard and D.E. Clapham, Simultaneous Near Ultraviolet and Visible Excitation Confocal Microscopy of Calcium Transients in Xenopus Oocytes. Use of Aequorin for [Ca2+]I Imaging: K.R. Robinson, T.J. Keating, and R. J. Cork, Inexpensive Techniques for Measuring [Ca2+]I Changes Using a Photomultiplier Tube. A.L. Miller, E. Karplus, and L.F. Jaffe, Imaging [Ca2+]I with Aequorin Using a Photon Imaging Detector. R. Rizzuto, M. Brini, and T. Pozzan, Targeting Recombinant Aequorin to Specific Intracellular Organelles. Index.

Reviews

This multiauthored book... provides theoretical and applied aspects of different established techniques for the measurement of [Ca2+]i at submicromolar concentration. Each chapter is organized, keeping in view the needs of a person working in the laboratory, by giving the detailed methodology required for the implementation of that particular technique, and all the chapters are well referenced...The book provides a pragmatic approach for the estimation of cytosolic [Ca2+]i and also provides a means for studying the spatial and temporal resolution of [Ca2+]i. The book achieves the stated goal in the preface, 'this material will be very useful to the investigator who wants to apply any of these methods to his or her research. --RAMESH BHALLA, University of Iowa, Iowa City The book achieves the stated goal in the preface, this material will be very useful to the investigator who wants to apply any of these methods to his or her research. --MOLECULAR & CELLULAR NEUROSCIENCES

Author Information

University of California, Santa Barbara, USA

Tab Content 6

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