3D Cell Culture: Methods and Protocols

Author:   John W. Haycock
Publisher:   Humana Press Inc.
Edition:   2011
Volume:   695
ISBN:  

9781607619833


Pages:   343
Publication Date:   02 November 2010
Format:   Hardback
Availability:   In Print   Availability explained
This item will be ordered in for you from one of our suppliers. Upon receipt, we will promptly dispatch it out to you. For in store availability, please contact us.

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3D Cell Culture: Methods and Protocols


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Full Product Details

Author:   John W. Haycock
Publisher:   Humana Press Inc.
Imprint:   Humana Press Inc.
Edition:   2011
Volume:   695
Dimensions:   Width: 17.80cm , Height: 2.50cm , Length: 25.40cm
Weight:   0.867kg
ISBN:  

9781607619833


ISBN 10:   1607619830
Pages:   343
Publication Date:   02 November 2010
Audience:   Professional and scholarly ,  Professional & Vocational
Format:   Hardback
Publisher's Status:   Active
Availability:   In Print   Availability explained
This item will be ordered in for you from one of our suppliers. Upon receipt, we will promptly dispatch it out to you. For in store availability, please contact us.

Table of Contents

3D Cell Culture: A Review of Current Approaches and Techniques.- Scaffolds for Tissue Engineering and 3D Cell Culture.- Tracking Nanoparticles in Three Dimensional Tissue Engineered Models Using Confocal Laser Scanning Microscopy.- Using Immuno-Scanning Electron Microscopy for the Observation of Cell Adhesion Interaction at the Nano- and Microscale in S-phase Cells.- 3D Sample Preparation for Orthopedic Tissue Engineering Bioreactors.- Quantification of mRNA Using Real-Time PCR and Western Blot Analysis of MAPK Events in Chondrocyte/Agarose Constructs.- Genetic Modification of Chondrocytes Using Viral Vectors.- Stem Cell and Neuron Co-Cultures for the Study of Nerve Regeneration.- Production of Tissue Engineered Skin and Oral Mucosa for Clinical and Experimental Use.- Three-Dimensional Alignment of Schwann Cells Using Hydrolysable Microfiber Scaffolds: Strategies for Peripheral Nerve Repair.- Encapsulation of Human Articular Chondrocytes into 3D Hydrogel: Phenotype and Genotype Characterization.- Micro-Structured Materials and Mechanical Cues in 3D Collagen Gels.- Organotypic and 3D Reconstructed Cultures of the Human Bladder and Urinary Tract.- Ex Vivo Organ Culture of Human Hair Follicles: A Model Epithelial - Neuroectodermal - Mesenchymal Interaction System.- Human Endothelial and Osteoblast Co-Cultures on 3D Biomaterials.- Assessment of Nanomaterials Cytotoxicity and Internalization.- Practical Aspects of OCT Imaging in Tissue Engineering.- Osteogenic Differentiation of Embryonic Stem Cells in 2D and 3D Culture.- 3D Structuring of Biocompatible and Biodegradable Polymers via Stereolithography.- Alvetex®: Polystyrene Scaffold Technology for Routine Three Dimensional Cell Culture.

Reviews

From the reviews: This book is an excellent resource to help researchers find practical information to enable them to develop three-dimensional cell culture systems. ... anyone who wishes to enter this field should be able to use this book to develop their own bespoke 3D system. In conclusion, I would recommend this book as being very useful to a wide audience ... . will be essential for graduate students and postdocs as well as to investigators wishing to expand their work into 3D cell culture and tissue engineering. (Julian B. Chaudhuri, ChemBioChem, Vol. 12, 2011) A necessary book for all who use cell culture. No other words can better tell the reader about this compendium of the most innovative culture techniques we hold to try to reach the ultimate goal of cell culture ... . For those who wish to enter the field, some introductory reviews explain the present-day challenges in 3D culture while more detailed protocols talk about bioreactors and agarose constructs. (Carlo Alberto Redi, European Journal of Histochemistry, Vol. 55, 2011)


From the reviews: This book is an excellent resource to help researchers find practical information to enable them to develop three-dimensional cell culture systems. ! anyone who wishes to enter this field should be able to use this book to develop their own bespoke 3D system. In conclusion, I would recommend this book as being very useful to a wide audience ! . will be essential for graduate students and postdocs as well as to investigators wishing to expand their work into 3D cell culture and tissue engineering. (Julian B. Chaudhuri, ChemBioChem, Vol. 12, 2011) A necessary book for all who use cell culture. No other words can better tell the reader about this compendium of the most innovative culture techniques we hold to try to reach the ultimate goal of cell culture ! . For those who wish to enter the field, some introductory reviews explain the present-day challenges in 3D culture while more detailed protocols talk about bioreactors and agarose constructs. (Carlo Alberto Redi, European Journal of Histochemistry, Vol. 55, 2011)


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