Overview

This volume covers the latest techniques and strategies used in multi-photon excitation (MPE) microscopy. Chapters in this book cover the fundamentals of MPE microscopy as applied to both in vitro and in vivo experimental systems; information on how to combine MPE microscopy with targeted electrophysiological recordings, calcium imaging, and transmembrane voltage imaging; methods to investigate cellular and large-scale neural morphology; signaling in astrocytes; and ways to use MPE microscopy to study the retina. In Neuromethods series style, chapters include the kind of detail and key advice from the specialists needed to get successful results in your laboratory. Comprehensive and thorough, Multiphoton Microscopy is a valuable resource for both expert and novice researchers interested in expanding their knowledge and research in this rapidly developing field.

Full Product Details

Author:   Espen Hartveit
Publisher:   Humana Press Inc.
Imprint:   Humana Press Inc.
Edition:   1st ed. 2019
Volume:   148
Weight:   0.928kg
ISBN:  

9781493997015

ISBN 10:   1493997017
Pages:   364
Publication Date:   01 November 2019
Audience:   Professional and scholarly ,  Professional & Vocational
Format:   Hardback
Publisher's Status:   Active
Availability:   Manufactured on demand  
We will order this item for you from a manufactured on demand supplier.

Table of Contents

Building a Two-Photon Microscope is Easy.- Using Multiphoton Imaging for Targeted Electrophysiological Recording and Live Cell Imaging of Fluorescently Labeled Neurons from Isolated Retinas.- Two-Photon Neurotransmitter Uncaging for the Study of Dendritic Integration.- Multiphoton Glutamate Uncaging to Study Structural and Functional Plasticity of Dendritic Spines.- Two-Photon Fluorescence Imaging of Visually Evoked Glutamate Release using iGluSnFR in the Mouse Visual System.- Imaging Neuronal Signal Transduction using Multiphoton FRET-FLIM.- Combining Multiphoton Excitation Microscopy with Fast Microiontophoresis to Investigate Neuronal Signaling.- Multiphoton Excitation Microscopy for Reconstruction and Analysis of Single Neuron Morphology.- Serial Multiphoton Tomography and Analysis of Volumetric Images of the Mouse Brain.- Studying a Light Sensor with Light: Multiphoton Imaging in the Retina.- Multiphoton Ca2+ Imaging of Astrocytes with Genetically Encoded Indicators Delivered by a Viral Approach.- Two-Photon Imaging of Dendritic Calcium Dynamics In Vivo.- Voltage Imaging with ANNINE Dyes and Two-Photon Microscopy.- High-Speed, Random-Access Multiphoton Microscopy for Monitoring Synaptic and Neuronal Activity in 3D in Behaving Animals.

Reviews

“I recommend this exciting book, replete with full-color images, because of its comprehensive, tested protocols for the study of live neural tissue at the cellular and the subcellular levels.” (Barry R. Masters, Optics & Photonics News, osa-opn.org, March 5, 2020)

I recommend this exciting book, replete with full-color images, because of its comprehensive, tested protocols for the study of live neural tissue at the cellular and the subcellular levels. (Barry R. Masters, Optics & Photonics News, osa-opn.org, March 5, 2020)

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