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OverviewFull Product DetailsAuthor: Benjamin F. Lasseter (Christopher Newport University, VA, USA)Publisher: Taylor & Francis Ltd Imprint: CRC Press Weight: 0.426kg ISBN: 9781138589926ISBN 10: 1138589926 Pages: 188 Publication Date: 07 October 2019 Audience: College/higher education , Undergraduate Format: Hardback Publisher's Status: Active Availability: In Print  This item will be ordered in for you from one of our suppliers. Upon receipt, we will promptly dispatch it out to you. For in store availability, please contact us. Table of ContentsAuthor Bio Chapter 1 Buffers Chapter 2 Assays Chapter 3 Protein Concentration Chapter 4 ELISA Chapter 5 Salting Out Proteins and Other Biomolecules Chapter 6 A Discussion of Isoelectric Point and Effective Charge Chapter 7 Column Chromatography Chapter 8 Michaelis–Menten Kinetics Chapter 9 Protein Purification Chapter 10 Polyacrylamide Gels Chapter 11 In Silico Biochemistry: The Evolution of Globins Chapter 12 Growing Crystals of Hemoglobin Chapter 13 Enzyme Inhibition Chapter 14 Multisubstrate Kinetics Chapter 15 Fluorescence and Denaturation Chapter 16 Fluorescence Studies of Ligand Binding Chapter 17 DNA Restriction Digests Chapter 18 Western Blotting IndexReviewsAuthor Lasseter (biochemistry, Christopher Newport Univ.) has written several laboratory manuals for general chemistry. This manual provides 18 chapters, each dealing with a single biochemical laboratory procedure. Several chapters end with a goal-assemble the methods and materials to use-and an optional goal-use and test materials after they are prepared. All chapters end with pre-lab and post-lab questions as well as references. Topics covered include buffers, assays, protein concentrations, ELISA (Enzyme-Linked ImmunoabSorption Assay), salting out biomolecules, isoelectric points and effective charges, column chromatography, Michaelis-Menten kinetics (biochemical reaction rates), protein purification, polyacrylamide gels and their uses, computational biochemistry (the example used is the evolution of globins), growing crystals of hemoglobin, enzyme inhibition, multisubstrate kinetics, fluorescence and denaturation, fluorescence studies of ligand binding, DNA restriction digests (i.e., determination of sites), and western blotting (combined use of ELISA and immunostaining). In the current pandemic time, applicability of any lab manual given total shutdown of academic labs followed by only partial reopening is problematic, yet resources are available for teaching science labs under these conditions. Undergraduate students of biochemistry and their instructors will appreciate this book given the appropriate conditions for use. Summing Up: Recommended. Lower- and upper-division undergraduates. Students enrolled in two-year technical programs. Graduate students and faculty. --R. E. Buntrock, independent scholar, CHOICE Author Lasseter (biochemistry, Christopher Newport Univ.) has written several laboratory manuals for general chemistry. This manual provides 18 chapters, each dealing with a single biochemical laboratory procedure. Several chapters end with a goal-assemble the methods and materials to use-and an optional goal-use and test materials after they are prepared. All chapters end with pre-lab and post-lab questions as well as references. Topics covered include buffers, assays, protein concentrations, ELISA (Enzyme-Linked ImmunoabSorption Assay), salting out biomolecules, isoelectric points and effective charges, column chromatography, Michaelis-Menten kinetics (biochemical reaction rates), protein purification, polyacrylamide gels and their uses, computational biochemistry (the example used is the evolution of globins), growing crystals of hemoglobin, enzyme inhibition, multisubstrate kinetics, fluorescence and denaturation, fluorescence studies of ligand binding, DNA restriction digests (i.e., determination of sites), and western blotting (combined use of ELISA and immunostaining). In the current pandemic time, applicability of any lab manual given total shutdown of academic labs followed by only partial reopening is problematic, yet resources are available for teaching science labs under these conditions. Undergraduate students of biochemistry and their instructors will appreciate this book given the appropriate conditions for use. Summing Up: Recommended. Lower- and upper-division undergraduates. Students enrolled in two-year technical programs. Graduate students and faculty. --R. E. Buntrock, independent scholar, CHOICE Author InformationDr. Benjamin F. Lasseter earned his Ph.D. in Biochemistry from Texas A&M University in 2003. He briefly assisted in the production of history textbooks for the Catholic Schools Textbook Project before teaching at a high school in Louisiana. He moved on to Christopher Newport University in 2011, where he teaches the biochemistry labs. He mixes analytical chemistry with pure biology to study environmental impacts of metal pollution. Tab Content 6Author Website:Countries AvailableAll regions |
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