Influenza Virus: Methods and Protocols

Author:   Yohei Yamauchi
Publisher:   Humana Press Inc.
Edition:   Softcover reprint of the original 1st ed. 2018
Volume:   1836
ISBN:  

9781493993635


Pages:   663
Publication Date:   10 December 2019
Format:   Paperback
Availability:   Manufactured on demand   Availability explained
We will order this item for you from a manufactured on demand supplier.

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Influenza Virus: Methods and Protocols


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Overview

This book provides researchers with widely used techniques for the study of virology, focusing on molecular biology and imaging to encourage mechanistic investigation of virus-host interactions. Chapters detail a broad range of methods from diagnosis, virus propagation, proteomics, haploid screening, lentiviral screening, virus entry, single molecule RNA imaging, correlative light and electron microscopy (CLEM), EM, light-sheet microscopy, biochemistry, viral transcription, physiological infection models, animal models, in vivo imaging, antigenic evolution, immunology to mathematical modelling. Reviews cover general influenza, clinical trials, both sides of the gain-of-function debate, and computational modelling. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Cutting-edge and thorough, Influenza Virus: Methods and Protocols aims to motivate experienced researchers and newcomers in the field and improve our overall understanding of influenza.

Full Product Details

Author:   Yohei Yamauchi
Publisher:   Humana Press Inc.
Imprint:   Humana Press Inc.
Edition:   Softcover reprint of the original 1st ed. 2018
Volume:   1836
Weight:   1.274kg
ISBN:  

9781493993635


ISBN 10:   1493993631
Pages:   663
Publication Date:   10 December 2019
Audience:   Professional and scholarly ,  Professional & Vocational
Format:   Paperback
Publisher's Status:   Active
Availability:   Manufactured on demand   Availability explained
We will order this item for you from a manufactured on demand supplier.

Table of Contents

Standard Influenza protocols and surveillance 1. Influenza Virus isolation, preparation and titration 2. Vaccine selection and preparation 3. Isolation of clinical strains, sequencing, and reverse genetics 4. Diagnosis 5. Temporal-spatial analysis and real time surveillance of virus outbreaks (i.e. Ebola) 6. How the general population, internet, SNS helps predict epidemics (Overview) Systems approach to discover Influenza virus-host interactions 7. Integrated Omics and Systems Virology (Overview) 8. Computational Biology of Infectious Diseases and study of virus evolution 9. Haploid Screening for host factors in virus infection 10. Proteomics, and affinity-MS virus interactome 11. Influenza Virus Replicon system in Yeast 12. Virus Receptor Hunting using Ligand Based Receptor Capture 13. Fully-automated screen using FDA-approved drug libraries Dissecting cell biology of Influenza infection 14. Cell Biology of influenza virus-host interactions (Overview) 15. Influenza virus entry assays in tissue culture cells 16. DC-SIGN mediated virus uptake analysis 17. In vitro assay to detect virus disassembly during endocytic transit 18. Endosome fractionation and detection of viral and cellular antigens 19. Virus-liposome fusion assays using cryo EM 20. Visualizing influenza capsid uncoating in the cytoplasm of cells using combined cryo EM, SEM, and aqueous AFM 21. Quantitative PCR based analysis of Influenza virus endocytic escape 22. Virus uncoating detection using beta lactamase fused VLPs 23. Purification of viral polymerase using MultiBac system for structural biology 24. Influenza polymerase transcription analysis - vRNA vs cRNA 25. Image Analysis: From Single Cell analysis to High throughput imaging (Overview) 26. Single molecule FISH of incoming and egressing influenza vRNPs 27. Live imaging of replication and egress using a GFP influenza virus 28. Virus fluorescent labeling for imaging and super-resolution microscopy 29. Cryo EM of influenza virus progeny during budding 30. Assessing virus growth in cells depleted of host factors with siRNA or CRISPR/Cas9 Influenza animal models, physiological systems, and immunology 31. Animal models: mice, ferrets, pigs, monkeys and CRISPR animals (Overview) 32. IAV in vivo imaging, virus titration and immune response analysis 33. MxA and interferon (Overview) 34. Clinical bacteria and viral co-infection studies 35. 3D ex vivo culture system to study virus infection and entry

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