Overview

This volume details cutting-edge protocols on the characterization of the genome, epigenome, proteome, metabolome and single-cell transcriptome of tumors and tumor-derived cultures. Chapters focus on subpopulations of cells with stem-like properties, laser capture microdissection, and modeling human glioma with human embryonic stem cells. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and cutting-edge, Glioblastoma: Methods and Protocols aims to support researchers seeking new and refined protocols to better decrypt this tumor. 

Full Product Details

Author:   Dimitris G. Placantonakis
Publisher:   Humana Press Inc.
Imprint:   Humana Press Inc.
Edition:   Softcover reprint of the original 1st ed. 2018
Volume:   1741
Weight:   0.493kg
ISBN:  

9781493985364

ISBN 10:   1493985361
Pages:   241
Publication Date:   04 June 2019
Audience:   Professional and scholarly ,  Professional & Vocational
Format:   Paperback
Publisher's Status:   Active
Availability:   Manufactured on demand  
We will order this item for you from a manufactured on demand supplier.

Table of Contents

Whole Genome Sequencing-based Discovery of Structural Variants in Glioblastoma.- Whole Genome DNA Methylation Analysis of Human Glioblastoma using Illumina BeadArrays.- Establishing Primary Human Glioblastoma Adherent Cultures from Operative Specimens.- Establishing Primary Human Glioblastoma Tumorsphere Cultures from Operative Specimens.- Isolation of GBM Stem Cells with Flow Cytometry.- Lentiviral Transduction of Primary Human Glioblastoma Cultures.- Selective Targeting of CD133-expressing Glioblastoma Stem Cells using Lentiviral Vectors.- Intracellular pH Measurements in Glioblastoma Cells using the pH-sensitive Dye BCECF.- Induction and Assessment of Hypoxia in Glioblastoma Cells in vitro.- Metabolomic Analysis of Glioma Cells Using Nanoflow Liquid Chromatography-tandem Mass Spectrometry.- Sample Preparation for Relative Quantitation of Proteins Using Tandem Mass Tags (TMT) and Mass Spectrometry (MS).- Single-Cell RNA Sequencing of Glioblastoma Cells.- Evaluation of Radioresponse and Radiosensitizers in Glioblastoma Organotypic Cultures.- Orthotopic Patient-derived Glioblastoma Xenografts in Mice.- Bioluminescent In Vivo Imaging of Orthotopic Glioblastoma Xenografts in Mice.- Evaluation of Vascularity, Blood Perfusion, and Oxygen Tension in Tumor Xenografts with Fluorescent Microscopy.- Probing Glioblastoma Tissue Heterogeneity with Laser Capture Microdissection.- Flow Cytometric Identification of Tumor-infiltrating Lymphocytes from Glioblastoma.- Modeling Glioma with Human Embryonic Stem Cell-derived Neural Lineages. 

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