Overview

This volume covers various assays and techniques that have been developed to study and characterize the cell migration in vitro, ex vivo, and in vivo. The chapters in this book present readers with the latest protocols to observe, quantify, and control cell migration. Some of the topics explored in this book are: migration in confined environments, microfluidic devices, optogenetics, chemotaxis, electrotaxis, detection of migrasomes, migration of Q cells in Caenorhabditis elegans, of Drosophila macrophages, optogenetics of cell migration, intravital imaging. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Cutting edge and comprehensive, Cell Migration: Methods and Protocols is a valuable resource for anyone interested in learning more about this expanding field.

Full Product Details

Author:   Alexis Gautreau
Publisher:   Humana Press Inc.
Imprint:   Humana Press Inc.
Edition:   Softcover reprint of the original 1st ed. 2018
Volume:   1749
Weight:   0.795kg
ISBN:  

9781493992614

ISBN 10:   1493992619
Pages:   405
Publication Date:   10 December 2019
Audience:   Professional and scholarly ,  Professional & Vocational
Format:   Paperback
Publisher's Status:   Active
Availability:   Manufactured on demand  
We will order this item for you from a manufactured on demand supplier.

Table of Contents

Random Migration Assays of Mammalian Cells and Quantitative Analyses of Single Cell Trajectories.- Directional Collective Migration in Wound Healing Assays.- An In Vitro System to Study the Mesenchymal-to-Amoeboid Transition.- An In Vitro System to Study the Epithelial-Mesenchymal Transition In Vitro.-  Detection of Migrasomes.- 3D Endothelial Cell Migration.- Transmigration of Leukocytes across Epithelial Monolayers.- Evaluation of Tumor Cell Invasiveness In Vivo: The Chick Chorioallantoic Membrane Assay.- Analysis of Invasion Dynamics of Matrix-Embedded Cells in a Multi-Sample Format.- Using Systems Microscopy to Understand the Emergence of Cell Migration from Cell Organization.- Neuronal Precursor Migration in Ex Vivo Brain Slice Culture.- In Vitro Models to Analyze the Migration of MGE-Derived Interneurons.- Cell Migration in Tissues: Explant Culture and Live Imaging.- Intravital Imaging of Tumor Cell Motility inthe Tumor Microenvironment Context.- Using the Zebrafish Embryo to Dissect the Early Steps of Metastasis Cascade.- Analysis of In Vivo Cell Migration in Mosaic Zebrafish Embryos.- Analysis of Cell Shape and Cell Migration of Drosophila Macrophages In Vivo.- Migration of Q Cells in Caenorhabditis elegans.- Imaging the Molecular Machines that Power Cell Migration.- A Biologist-Friendly Method to Analyze Cross-Correlation between Protrusion Dynamics and Membrane Recruitment of Actin Regulators.- Using Single Protein Tracking to Study Cell Migration.- Optogenetics of Cell Migration.- Electrotaxis: Cell Directional Movement in Electric Fields.- Analysis of Random Migration of Dictyostelium Amoeba in Confined or Unconfined Environments.- Neutrophil Chemotaxis in One Droplet of Blood using Microfluidic Assays.- Leukocyte Migration and Deformation in Collagen Gels and Micro-Fabricated Constrictions.- Microfluidic Devices for Examining the Physical Limits of Migration in Confined Environments.- Controlling Confinement and Topology to Study Collective Cell Behaviors.

Reviews

This book presents several techniques to study cells migration in vivo, in vitro, ex vivo and with different model systems to dissect many of the biochemical and biophysical properties (at both molecular and cellular levels) involved in the dynamics of migration and cell-to-cell communication. ... the editor of this book did a great job in collecting contributions where several animal models are presented. (Manuela Monti, European Journal of Histochemistry, Vol. 63 (01), 2019)

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