Overview

The third edition of this book provides a large number of laboratory protocols that are intended for basic and clinical bone researchers. Chapters are organized into five parts covering methods for isolation, ex vivo models of bone organ and cell co-culture system, biochemical procedures, animal models of bone remodeling, and rodent models on Rheumatoid arthritis and osteoarthritis. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and cutting-edge Bone Research Protocols: Methods and Protocols, Third Edition aims to extensive list of protocols for anyone interested in bone research.

Full Product Details

Author:   Aymen I. Idris
Publisher:   Humana Press Inc.
Imprint:   Humana Press Inc.
Edition:   3rd ed. 2019
Volume:   1914
Weight:   1.481kg
ISBN:  

9781493989966

ISBN 10:   1493989960
Pages:   670
Publication Date:   08 February 2019
Audience:   Professional and scholarly ,  Professional & Vocational
Format:   Hardback
Publisher's Status:   Active
Availability:   Manufactured on demand  
We will order this item for you from a manufactured on demand supplier.

Table of Contents

Part I: In Vitro Culture of Bone Cells 1. Isolation and Generation of Osteoclasts Nadia Rucci, Alberta Zallone, and Anna Teti 2. Isolation and Generation of Osteoblasts Ines P Perpetuo, Lucie E Bourne, and Isabel R Orriss 3. Isolation, Purification, Generation, and Culture of Osteocytes Jonathan H Gooi, Ling Yeong Chia, Christina Vrahnas, and Natalie A Sims 4. Isolation, Differentiation, and Characterisation of Human Bone Marrow Stem Cells In Vitro and In Vivo Janos Kanczler, Rahul S. Tare, Peter D. Mitchell, Patrick Stumpf, Timothy J. Noble, Cameron Black, and Richard O.C. Oreffo 5. 2d and 3d In Vitro Co-Culture For Cancer and Bone Cell Interaction Studies Silvia Marino, Ryan T. Bishop, Danielle De Ridder, Jesus Delgado-Calle, and Michaela R Reagan 6. Using Cell and Organ Culture Models to Analyse Responses of Bone Cells to Mechanical Stimulation Soraia P Caetano-Silva, Astrid Novicky, Behzad Javaheri, Simon Cf Rawlinson, and Andrew A Pitsillides Part II: Ex Vivo Culture of Bone Cells 7. The ex vivo Organ Culture of Bone Katherine A. Staines, Genevieve Brown, and Colin Farquharson 8. Ex-Vivo Model Systems of Cancer-bone Cell Interactions Erdjan Salih 9. Mechanical Properties of Bone ex vivo Simon R. Goodyear and Richard M. Aspden Part III: Ex Vivo Culture of Bone Cells 10. Analysis of Signalling Pathways by Western Blotting and Immunoprecipitation Silvia Marino and Aymen I. Idris 11. Analysis of Transcriptional Regulation in Bone Cells Huilin Jin, Antonia Sophocleous, Asim Azfer, and Stuart H Ralston 12. Analysis of mRNA, miRNA, and DNA in bone cells by RT-qPCR and In situ Hybridization Brice Moukengue, Jerome Amiaud, Camille Jacques, Celine Charrier, Benjamin Ory, and Francois Lamoureux Part IV: In Vivo Models of Bone 13. Ovariectomy/Orchiectomy in Rodents Antonia Sophocleous and Aymen I. Idris 14. In Vivo Models of Rheumatoid Arthritis Bettina Groetsch, Aline Bozec and Georg Schett 15. Osteoarthritis Mouse Model of Destabilisation of the Medial Meniscus Antonia Sophocleous and Carmen Huesa 16. Models of Prostate Cancer Bone Metastasis Sun Hee Park, Matthew Robert Eber, andYusuke Shiozawa 17. Animal Models of Breast Cancer Bone Metastasis Claudia Tulotta, Arwin Groenewoud, B. Ewa Snaar-Jagalska, and Penelope Ottewell 18. Murine Models of Bone Sarcomas Camille Jacques, Nathalie Renema, Benjamin Ory,Carl R. Walkley, Agi E. Grigoriadis, and Dominique Heymann 19. Mouse Models of Melanoma Bone Metastasis OEzge Uluckan 20. Animal Models of Multiple Myeloma Sam W.Z. Olechnowicz and Claire M. Edwards 21. The Calvarial Injection Assay Robert J. van `t Hof 22. In Vivo Models of Mechanical Loading Behzad Javaheri, Nathalie Bravenboer, Astrid D. Bakker, Albert van der Veen, Roberto Lopes de Souza, Leanne Saxon, and Andrew A. Pitsillides 23. Animal Models for the Study of Bone Derived Pain Austen L. Thompson, Tally M. Largent-Milnes, and Todd W. Vanderah Part V: Microscopical and Imaging Techniques 24. Histomorphometry in Rodents Reinhold G. Erben and Martin Gloesmann 25. Immunostaining of Skeletal Tissues Anke J. Roelofs and Cosimo De Bari 26. Techniques for the Study of Apoptosis in Bone Val Mann and Brendon Noble 27. Live Cell Imaging of Bone Cell and Organ Cultures Sarah L. Dallas, Patricia A. Veno, and LeAnn M. Tiede-Lewis 28. Analysis of Bone Architecture in Rodents using micro Computed Tomography Robert J. van 't Hof and Enrico Dall'Ara 29. Bone Measurements by Peripheral Quantitative Computed Tomography in Rodents Jurg A. Gasser and Johannes Willnecker 30. Quantitative X-ray Imaging of Mouse Bone by Faxitron Natalie C Butterfield, John G Logan, Julian Waung, Graham R Williams, and JH Duncan Bassett 31. Scanning Electron Microscopy of Bone Alan Boyde 32. Transmission Electron Microscopy of Bone Vincent Everts, Anneke Niehof, Wikky Tigchelaar-Gutter, and Wouter Beertsen 33. Tracking Cancer Cells Colonisation in Rodent Bone Using Two-photon Microscopy Gloria Allocca and Ning Wang 34. Fourier Transform Infrared Imaging of Bone Eleftherios P. Paschalis 35. Raman Microscopy and Bone Simon R. Goodyear and Richard M. Aspden

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