Overview

Biorthogonal Tagging, Volume 731 in the Methods in Enzymology series, highlights new advances in the field, with this new volume presenting interesting chapters such as Staudinger Ligations and Related Reactions: Scope and Applications in Chemical Biology, Strain-Promoted Bioorthogonal Cycloadditions to Investigate Sialyltransferases Promiscuity Towards Unnatural Sialic Acids, Practical Guidelines for the Bioorthogonal AMI–Isonitrile Ligation, Nitrones and Other Dipoles in Bioorthogonal Chemistry Applications, 1,2-Aminothiol-Specific Conjugation for Dual-Color Fluorescent Labeling via Ultrafast TAMMs, Genetic Incorporation of Bicyclononyne for Cell-Surface Protein Labeling via Tetrazine Ligation, and much more. Additional chapters cover Genetic Code Expansion for Single Noncanonical Amino Acid Incorporation into SARS-CoV-2 Omicron Spike on Amber-Free Lentiviral Particles, Bioorthogonal Click-Chemistry Labeling and Visualization of Dual Noncanonical Amino Acid–Incorporated HIV-1 Env on Intact Virions, Studying Bacterial Glycans with Azidosugars and Bioorthogonal Chemistry, Bioorthogonal Tagging of DNA and RNA, Efficient Labeling of Pathogenic Fungal Chitin with Bioorthogonal Probes, and many more timely topics.

Full Product Details

Author:   David Christianson (University of Pennsylvania, USA) ,  Karen N. Allen (Department of Chemistry, Metcalf Center for Science and Engineering, Boston University, Boston, MA, USA) ,  Adam Urbach
Publisher:   Elsevier Science Publishing Co Inc
Imprint:   Academic Press Inc
Weight:   0.450kg
ISBN:  

9780443432248

ISBN 10:   0443432244
Pages:   258
Publication Date:   01 June 2026
Audience:   Professional and scholarly ,  Professional & Vocational
Format:   Hardback
Publisher's Status:   Active
Availability:   In Print  
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Table of Contents

1. Staudinger Ligations and Related Reactions: Scope and Applications in Chemical Biology Stefan Bräse, Juliane Ott and Ute Schepers 2. Strain-Promoted Bioorthogonal Cycloadditions to Investigate Sialyltransferases Promiscuity Towards Unnatural Sialic Acids Frederic Friscourt and Zoeisha Chinoy 3. Practical Guidelines for the Bioorthogonal AMI–Isonitrile Ligation Helma Wennemers 4. Nitrones and Other Dipoles in Bioorthogonal Chemistry Applications John Paul Pezacki 5. 1,2-Aminothiol-Specific Conjugation for Dual-Color Fluorescent Labeling via Ultrafast TAMMs Yu-Hsuan Tsai, Linlin Wang, Gaocan Dai and Sicong Yao 6. Genetic Incorporation of Bicyclononyne for Cell-Surface Protein Labeling via Tetrazine Ligation Yu-Hsuan Tsai, Yawei Liu, Lei Zhang and Sicong Yao 7. Genetic Code Expansion for Single Noncanonical Amino Acid Incorporation into SARS-CoV-2 Omicron Spike on Amber-Free Lentiviral Particles Maoliln Lu 8. Bioorthogonal Click-Chemistry Labeling and Visualization of Dual Noncanonical Amino Acid–Incorporated HIV-1 Env on Intact Virions Maoliln Lu 9. Studying Bacterial Glycans with Azidosugars and Bioorthogonal Chemistry Danielle H. Dube, Aniqa Chowdhury, Divya Bhargava and Karen Moulton 10. Bioorthogonal Tagging of DNA and RNA Seergazhi G. Srivatsan 11. Efficient Labeling of Pathogenic Fungal Chitin with Bioorthogonal Probes Catherine L. Grimes 12. Dendritic Cell Metabolic Labeling and Targeting Hua Wang 13. Orthogonal Crosslinking of Monobodies Using a Genetically Encoded β-Lactam Amino Acid Qing Lin 14. Bioorthogonal Chemical Labeling of Endogenous Receptors in the Live Mouse Brain Itaru Hamachi 15. Organelle-Directed Metabolic Glycan Labeling Shou-Fa Han, Shixiong Wen, Yilong Shi, Enkang Zhang and Jiahuai Han 16. Bioorthogonal Click Labeling of Proteins for Molecular Resolution Fluorescence Imaging Markus Sauer 17. Molecular Engineering of Activatable Cyclopropenes for Bioorthogonal Labeling Scott T. Laughlin 18. Pyrone and Pyranthione Scaffolds for Click-to-Release Biological Gas Delivery Scott T. Laughlin 19. Click and Release through Bioorthogonal Hydroamination Justin Kim, Tyler J Brown and Yiming Guo 20. Site-Specific Bioorthogonal Labeling of Antibodies Lai-Xi Wang 21. inCu-click: Enabling Copper-Catalyzed Click Chemistry Inside Living Cells Sara H. Rouhanifard 22. Bioorthogonal Photocatalytic Proximity Labeling for Quantitative Mapping of Cell–Cell Interactions Xinyuan Fan, Yan Zhang, Nan Zhou and Fuhu Guo 23. Bioorthogonal Labeling of Sialic Acid Isomers for Imaging of Glycoconjugates Using Mass Spectrometry and Microscopy Richard R. Drake

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Author Information

After completing studies for the A.B., A.M., and Ph.D. degrees in chemistry at Harvard University, David W. Christianson joined the faculty of the University of Pennsylvania, where he is currently the Roy and Diana Vagelos Professor in Chemistry and Chemical Biology. At Penn, Christianson’s research focuses on the structural and chemical biology of the zinc-dependent histone deacetylases as well as enzymes of terpene biosynthesis. His research accomplishments have been recognized by several awards, including the Pfizer Award in Enzyme Chemistry and the Repligen Award in Chemistry of Biological Processes from the American Chemical Society, a Guggenheim Fellowship, and the Elizabeth S. and Richard M. Cashin Fellowship from the Radcliffe Institute for Advanced Study at Harvard University. Christianson is also a dedicated classroom teacher, and his accomplishments in this regard have been recognized by the Lindback Award for Distinguished Teaching at Penn and a Rhodes Trust Inspirational Educator Award from Oxford University. Christianson has also held visiting professorships in the Department of Biochemistry at Cambridge University and the Department of Chemistry and Chemical Biology at Harvard University. Christianson has served with Prof. Anna Pyle as Co-Editor-in-Chief of Methods in Enzymology since 2015. Dr. Karen N. Allen works at the Department of Chemistry of the Boston University, the Metcalf Center for Science and Engineering

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